with starch Iodine changes color when it connects to starch See Page 35 in Lab

# With starch iodine changes color when it connects to

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with starch Iodine changes color when it connects to starch! See Page 35 in Lab Manual for More

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VITAMIN C ASSAY What do we mean by concentration? Mass per Volume mg/ml Standard Curve Water (0 mg/ml Vitamin C) Standard solutions (0.3, 0.6, 1.0 mg/ml Vitamin C) When we know a value on one axis, we can find the value it corresponds with on the other axis! 0 mg/ml 0.6 mg/ml 0.3 mg/ml Exampl e Only! K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
PROCEDURE (SEE PG. 23 FOR THOROUGH INSTRUCTIONS! NUMBERING IS A BIT DIFFERENT HERE.) 1. Using the key on Pg. 23, which juices do you have available? 2. Which juice do you think has the highest concentration of Vitamin C? This is your hypothesis! 3. Set up a table to record your data 4. Test distilled water (we will do as a class) 5. Test each Vitamin C standard. *REMEMBER TO COUNT ALL DROPS* (we will do as a class) 6. Test each juice sample. 7. Construct a standard curve using water & Vit. C standards. Determine the concentration of Vitamin C in each juice sample. 8. Write a conclusion – do your results support your hypothesis? K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018

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TEST TUBES Row 1 Starch Iodine Row 2 DI Water 0.3 mg/ml Vit C 0.6 mg/ml Vit C 1.0 mg/ml Vit C Juice 1 Juice 2 Juice 3 Row 3 Labeling E1 E2 E3 E4 E5 E6 E7 Row 3 After Experimen t 25 drops water 2 drops starch Iodine 25 drops 0.3 VitC 2 drops starch Iodine 25 drops 0.6 VitC 2 drops starch Iodine 25 drops 1.0 VitC 2 drops starch Iodine 25 drops Juice 1 2 drops starch Iodine 25 drops Juice 2 2 drops starch Iodine 25 drops Juice 3 2 drops starch Iodine K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
Concentration of Vitamin C (mg/ml) Iodine (drops) 0 1 0.3 0.6 5 10 15 Exampl e Only! 13 drops of iodine indicates a solution has about 0.85 mg/ml Vitamin C K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 The more drops of iodine until a color change, the higher the concentrat ion of

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K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 Concentration of Vitamin C (mg/ml) 0 0. 3 1 0.6 Juice 1 Juice 2 Juice 3
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