13ml ph 5 buffer solution 13 ml of ph 10 buffer

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13mL pH 5 buffer solution, 13 mL of pH 10 buffer solution, timer, a box of parafilm, and tissues if necessary. Procedures: To start with, all the test tubes and pipettes must be labeled to prevent any confusions and contaminations. Test tubes and pipettes that are reused from part 1 does not need to be relabel. Label one pair of pipette and test tube pH 5 buffer solution and another pair of test tube and pipette pH 10 buffer solution. Next, we have to fill up and prepare the labels pipettes and test tube with the corresponding solutions. For tube “SPNB”, it needs to be dispensed with 0.5 mL of “S” substrate (H 2 O 2 ), 0.5 mL of “P” indicator for product (guaiacol), and 6.0 mL of “NB” neutral buffer (water). Then cover the “SPNB” tube up with parafilm and gently mix the solution. For test tube “ENB” it need to allocated with 0.5 of “E” enzyme (turnip peroxidase) and 6.5 mL of pH 5 buffer solution. Again, cover the “ENB” tube with parafilm and gently mix the solutions. After the two test tubes are ready transfer the mixture from “SPNB” tube to the
“ENB” tube, and cover the test tube up with parafilm, gently mix up the solutions. Let the test tube sit still while you record your observations. Observations should also be record over the period of 5 minutes and in a 30 second interval and color changes should be compared to the color pallet given to you by your instructor. Repeat the same steps for pH 10 solution buffers. Calculate the rate of reaction and compare it to the baseline rate in part 1. Perform each experiment at least two times for accuracy (obtain more materials if necessary). Do not dispose the materials used in this lab it may be required during the next lab. Thoroughly clean the “SPNB” and “ENB” test tubes. Obtain data from other groups, calculate the average and plot it on to the graph. Data/Observations: Group Data: Color Intensity (Oxygen/Product Formation)(%) Time (mins) pH 5 Trial 1: pH 5 Trial 2: pH 5 Average: pH 10 Trial 1: pH 10 Trial 2: pH 10 Average: 0.5 30.0 10.0 20.0 10.0 0.0 5.0 1.0 40.0 30.0 35.0 10.0 0.0 5.0 1.5 50.0 30.0 40.0 20.0 0.0 10.0 2.0 60.0 40.0 50.0 20.0 10.0 15.0 2.5 70.0 40.0 55.0 20.0 10.0 15.0 3.0 70.0 50.0 60.0 20.0 20.0 20.0 3.5 80.0 50.0 65.0 20.0 20.0 20.0 4.0 80.0 60.0 70.0 20.0 20.0 20.0 4.5 80.0 70.0 75.0 20.0 20.0 20.0 5.0 90.0 80.0 85.0 30.0 30.0 30.0 * This data table shows that the reaction rate was not as fast as the reaction rate of the neutral
buffer (pH 7) environment, but the closer the pH level is to 7 the fast the reaction is. The pH level of 5 shows a noticeably rapid rate than the ph level of 10 because it's closer to 7* *The graph shows that the reaction rate is the highest at ph 5 when it’s closest to the pH level of 7. But at pH 10 which is farther away from pH 7 than pH 5 the reaction rate is slower. This demonstrates that the enzyme-catalyzed reaction would have higher rates when it’s pH level is closest to 7* Class Data: Color Intensity (Oxygen/Product Formation)(%) Time (mins) pH 3 pH 5 pH 6 pH 7 pH 8 pH 10 pH 11 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.5 10.0 10.0 10.0 20.0 10.0 0.0 0.0 1.0 10.0 10.0 20.0 40.0 20.0 10.0 10.0
1.5 10.0 20.0 50.0 50.0 30.0 10.0 10.0 2.0 20.0 20.0 60.0 60.0 40.0 10.0 10.0 2.5 20.0 20.0 70.0 70.0 50.0 10.0 10.0 3.0 20.0 40.0 70.0 80.0 70.0 20.0 10.0 3.5 20.0 50.0 70.0 80.0 80.0 20.0 10.0 4.0 20.0 50.0 80.0 80.0 80.0 20.0 10.0 4.5 30.0 60.0 80.0 80.0 80.0 20.0 10.0 5.0 30.0 70.0 80.0 90.0 80.0 30.0 20.0 *This data table shows that at pH level 7 the oxygen production is the most and the enzyme-

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