1 prepare four nutrient agar plates by following

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1. Prepare four nutrient agar plates by following Steps 1 – 6 of Experiment 1. 2. Slowly pour agar into the snap-cap tubes until each one contains approximately 2 mL of agar. The snap-cap has a 5 mL volume capacity; therefore, your snap-cap should be approximately 40% full. Note: The snap-cap tubes are referred to as “stab tubes” after the agar has been poured and gelled. 3. Snap the lid on the tubes and store them upright at room temperature until the agar has solidified and cooled (30 – 60 minutes). The tubes can be used immediately or stored at 4 °C or 39 °F. If stored, let warm to room temperature for approximately 1 hour before use.
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Note: Stab tubes should be stored vertically positioned in a 100 mL beaker to allow the agar to solidify properly. 4. Put on a pair of gloves, and retrieve the Petri dish that you reserved from Experiment 1. Locate three separate bacterial colonies. If possible, use colonies that have differing morphologies. Record your observations of the morphological characteristics of each colony in Table 2. 5. Sterilize your inoculation loop with your candle. a. Pour 70% isopropyl alcohol into a 100 mL beaker to a depth of 2 – 4 cm. Place the cap back on the bottle, and position it far out of the way. b. Light your candle, and set it aside. Be very cautious that your candle is safely positioned away from the beaker of isopropyl alcohol. c. Dip the inoculation loop into the isopropyl alcohol for 10 seconds. Once you’ve put the inoculation loop in the alcohol, keep it angled down so that no alcohol drips back onto your hand.
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  • Fall '19
  • Agar plate, Petri dish, Agar, Stab Tube

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