expression that shuttles between the cytoplasm and the cell membrane. You would like to find out how your protein is moving throughout the cell so you want to purify the proteins that are responsible for this movement. With the tools that you possess what might you do?a. prepare an affinity column using anti-GFP antibodies to isolate the GFP-expressing proteins and those that might be associated with them for subsequent identification. b. Run the cell lysates over an ion exchange column and then make a GFP crystal in order to determine its structure using X-ray diffraction c. Do a SDS-PAGE gel and identify all the fluorescent bandsd. only a and b would worke. none of the above
Save Answer10. (Points: 1) Eukaryotic mRNAs form circular forms through the binding of PABPI and eIF4 subunits. What is the functional significance of this circularization process?