ChannelsLecture

Sukharev et al conductance of mscl under tension

Info icon This preview shows pages 7–15. Sign up to view the full content.

View Full Document Right Arrow Icon
(Sukharev et al .)
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Conductance of MscL Under Tension Conductance of MscL Under Tension Electrophysiology measurements (patch clamping) lead to current vs membrane tension. Measurements reveal five distinct conductance substates. (Sukharev et al .)
Image of page 8
Consequences of Ion Channel Gating: Consequences of Ion Channel Gating: The Action Potential The Action Potential
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Ubiquitous Phenomenon of Ubiquitous Phenomenon of Mechanosensation Mechanosensation (Gillespie and Walker) Touch sensation in worm Mechanical response of hair cells The main point: mechanosensation is everywhere. Informational currency is electrical – detection is mechanical. Repetition of same motif – mechanical excitation results in transient flow of ions.
Image of page 10
Mechanosensitive Channels as Mechanosensitive Channels as Osmotic Pressure Relief Valves Osmotic Pressure Relief Valves (Perozo and Rees) Hierarchy of mechanically-gated channels. Properties of channel have been investigated using electrophysiology. Gating tension of MscL serves to avoid membrane rupture. MscL MscK MscS
Image of page 11

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
More on Osmotic Shock More on Osmotic Shock
Image of page 12
Coarse-Grained Descriptions of Coarse-Grained Descriptions of Macromolecular Structure Macromolecular Structure Description of biological structures can be undertaken from a variety of different perspectives. Two key ways of viewing structure are ribbon diagrams and all-atom descriptions.
Image of page 13

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Conformational Change During Conformational Change During Gating Gating Hypothesized structural pathway for opening the channel. Tilting of alpha helices and corresponding opening of the pore. Key Question: How does mechanical tension couple to the conformational change? What are the energetic consequences to the surrounding membrane as a result of channel opening?
Image of page 14
Image of page 15
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern