B, D5.Membrane protein subunit which spans between the intermebrane space and the mitochondrial matrix C6.ATP synthesis occurs in this subunit D7.ADP binds here D8.Rotor moves within this subunit to change its conformations and enable ATP synthesis Electrons flow around this subunit and create rotational force CABCD
2. (18 Points)Below is a diagram of electron flow in the mitochondria membrane. Use it to answer the questionsbelow. NOTE: each “location for electron flow” below has a single answer, but the letters below can be assigned tomore than one.Figure 5. Electron Flow in the mitochondrial membrane involved in the reduction of oxygen. Locations for electron flow 1.Location where fatty acids can also donate electrons E, F2.Location where inhibition of electron flow can stop electron flow from only NADH E3.Location where inhibition of electron flow completely stops the reduction of Oxygen and ATP generation D, C.4.Location where inhibition of electron flow can be artificially induced, even though electrons from both NADH and FAD are blocked B5.Location where electrons from both NADH and FADH are blocked without artificial induction of electron flow B6.Location where electrons from the TCA cycle directly enter electron flow from a membrane bound enzyme E, F7.Location where NADH in solution donates electrons for electron flow A8.Location where after inhibition, the P/O ratio would be 1.5 for FADH electrons B9.Location where after inhibition, the P/O ratio would most likely be less than 1.5 for electron flow D3.(14 Points)Please answer each question with T/F regarding mitochondrial respiration. ABCDEF
a.In experiments of isolated mitochondria, addition of ADP and Pi results in ATP production and increased O2utilization (respiration) [True]b.Increasing the concentration of ATP can directly stimulate the rate of mitochondrial oxygen utilization (respiration) [False]c.When the appropriate adenosine phosphate molecule increases mitochondrial oxygen utilization (respiration), it does so in a stochiometric fashion. [True]d.Inhibitors that disrupt proton flow and electron flow stop oxygen utilization (respiration) but not ATP synthesis. [False]e.Inhibitors that disrupt the proton gradient can still result in electron flow and oxygen utilization (respiration) [True]f.Nearly all the mitochondrial genes are present in the mitochondrial genome rather than cellular (nuclear) genome [False]g.The mitochondrial oxidases can pass electrons to oxygen and generate free radical species that are harmful to humans [True]APPENDIX. Appendix I.
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