B D 5 Membrane protein subunit which spans between the intermebrane space and

B d 5 membrane protein subunit which spans between

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B, D 5. Membrane protein subunit which spans between the intermebrane space and the mitochondrial matrix C 6. ATP synthesis occurs in this subunit D 7. ADP binds here D 8. Rotor moves within this subunit to change its conformations and enable ATP synthesis Electrons flow around this subunit and create rotational force C A B C D
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2. (18 Points) Below is a diagram of electron flow in the mitochondria membrane. Use it to answer the questions below. NOTE: each “location for electron flow” below has a single answer, but the letters below can be assigned to more than one. Figure 5. Electron Flow in the mitochondrial membrane involved in the reduction of oxygen. Locations for electron flow 1. Location where fatty acids can also donate electrons E, F 2. Location where inhibition of electron flow can stop electron flow from only NADH E 3. Location where inhibition of electron flow completely stops the reduction of Oxygen and ATP generation D, C. 4. Location where inhibition of electron flow can be artificially induced, even though electrons from both NADH and FAD are blocked B 5. Location where electrons from both NADH and FADH are blocked without artificial induction of electron flow B 6. Location where electrons from the TCA cycle directly enter electron flow from a membrane bound enzyme E, F 7. Location where NADH in solution donates electrons for electron flow A 8. Location where after inhibition, the P/O ratio would be 1.5 for FADH electrons B 9.Location where after inhibition, the P/O ratio would most likely be less than 1.5 for electron flow D 3.(14 Points) Please answer each question with T/F regarding mitochondrial respiration. A B C D E F
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a. In experiments of isolated mitochondria, addition of ADP and Pi results in ATP production and increased O 2 utilization (respiration) [True] b. Increasing the concentration of ATP can directly stimulate the rate of mitochondrial oxygen utilization (respiration) [False] c. When the appropriate adenosine phosphate molecule increases mitochondrial oxygen utilization (respiration), it does so in a stochiometric fashion. [True] d. Inhibitors that disrupt proton flow and electron flow stop oxygen utilization (respiration) but not ATP synthesis. [False] e. Inhibitors that disrupt the proton gradient can still result in electron flow and oxygen utilization (respiration) [True] f. Nearly all the mitochondrial genes are present in the mitochondrial genome rather than cellular (nuclear) genome [False] g. The mitochondrial oxidases can pass electrons to oxygen and generate free radical species that are harmful to humans [True] APPENDIX. Appendix I.
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