10 count the bands for each sample and note their

This preview shows page 59 - 63 out of 75 pages.

10. Count the bands for each sampleandnotetheirrelativelocation.Compareyourresultsthepredictions table and identify yourisolated plasmid.CAUTION: High Voltage. Do nottouch the equipment while it isplugged in and running.CAUTION: Wear UV protective goggles.51
APPENDIX AList of Risk Level 1 Organisms Used During the SemesterBacillus subtilisBacillus atropheausCitrobacter freundiiEscherichia coliDH5αEscherichia coliK12Enterobacter aerogenesEnterobacter cloacaeEnterococcus duransGeobacillus stearothermophilusHalobacterium salinarumMicrococcus luteusMicrococcus roseusPseudomonas fluorescensRhodococcus rhodochrousSaccharomyces cerevisiaeSarcina aurantiacaSerratia marcescensSpirillum volutansStaphylococcus epidermidisStaphylococcus saprophyticusStreptococcus salivarius52
APPENDIX BMicrobiology VocabularyAseptic Technique:A technique used to handle a sterile substance withoutintroducing contaminationColony:Visible mass of bacteria, derived from one single cell or CFUColony Forming Unit (CFU):Each colony consists of the progeny of a single cell.Isolated Colony:A colony that is NOT touching any other colonies on the growthmediumCulture:Bacteria or other living organisms growing in a tube, plate, flask, bottleor any format of mediumMixed Culture:Culture with a mixed population of microorganismsPure Culture:Culture that contains only one type of microorganismSubculture:A new culture made by transferring cells (colony) from a previousculture to fresh growth mediumIncubate:To hold a culture (solution) UNDER CONTROLLED CONDITIONSInoculate:To purposely introduce bacteria or other organisms into sterile mediumin order to grow and study themMedium (Media):Surface/Liquid that provides nutrients for growth of bacteria;contains ions, amino acids, carbohydrates, fats, vitamins, water (liquid, solid orsemi-solid form), may also contain other “enriching” ingredientsGeneral Purpose Medium:Provides general nutrition and supports a wide rangeof microorganisms.Selective Medium:Used toSELECT (ISOLATE)specific groups of bacteria(Include substrates to enhance the growth of one type, but inhibit the growth ofanother type of microorganism.) Selects for the growth of a group of organismswith a common trait e.g. antibiotic resistance.Differential Medium:Used toDIFFERENTIATE (TELL APART)different types ofrelated bacteria;Includes compounds that produce a characteristic change inthe appearance of bacterial growth and/or the medium surrounding thecolonies.Agar:A complex polysaccharide that can be added to any kind of medium to make itsolid.Remains solid at higher temperatures and few bacteria can digest it.53
Fluid Thioglycollate Broth:Used to cultivate anaerobic microorganisms. Strictaerobes tend to grow in a thin layer at the surface of the medium; obligateanaerobes will only grow in the portion below the oxidized layer.

Upload your study docs or become a

Course Hero member to access this document

Upload your study docs or become a

Course Hero member to access this document

End of preview. Want to read all 75 pages?

Upload your study docs or become a

Course Hero member to access this document

Term
Spring
Professor
N/A
Tags
TSA, Agar plate

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture