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MIC 101 Exam 1 Winter 2013

Underneath the stage and on top of the condenser is

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12. Underneath the Stage and on Top of the Condenser is yet another Glass Plate exactly the same Size as a Microscope Slide. Well, OK, this guy is another Microscope Slide (Soviet Optical Engineering was Solid, if not overly Imaginative). Black Paint has been applied to the Upper Surface of this Slide such that it has three clear Apertures, one with a Diameter of ~1.0 cm, one with a Diameter of ~0.75 cm, and one with a Diameter of ~0.5 cm. A ridiculously small, icky Plastic Lever on Top of the Condenser moves this Black-Painted Glass Plate back and forth such that the 1.0 cm, the 0.75 cm or the 0.5 cm Aperture can be positioned immediately above the Condenser. This Plastic Lever corresponds to which Control(s) on your MIC 101 Zeiss Microscope? A. Auxiliary Lens Lever B. Field Diaphragm Control C. Condenser Focus Knob D. Condenser Diaphragm Control
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MIC 101 Exam 1 Page 9 13. The Refractive Index is the Ratio of the Velocity of Light in a Vacuum to its Velocity in a given Substance. • The Refractive Index for Air is 1.0008 (basically 1.0). • The Refractive Index for Water is 1.33, although it varies with Temperature, a Variation you can see as a Glass Beaker of Water produces Schlieren (German= streaks) as it heats-up over a Bunsen Burner. • The Refractive Index for most Biological Specimens is also 1.33, which explains why we have to use Stains to see most Biological Specimens. • The Refractive Index for Glass is 1.52. • The Refractive Index for Quartz is 1.55 • The Refractive Index for Diamonds 2.417 (which is very different from that of either Glass or Quartz) What is the Refractive Index of our Immersion Oil? 14. Your Lab Partner prepares Smears of (Left to Right) Bacillus cereus , Staphylococcus epidermidis and E. coli on a single Slide, allows the Smears to Air Dry and then Heat Fixes these Smears. Your Lab Partner then floods the Slide with Crystal Violet for one (1) Minute, rinses with DI Water for an incredibly long Period of Time, floods the Slide with Iodine for one (1) Minute and again rinses with DI Water for just about Forever. Finally, your Lab Partner blots this Slide dry with our Bibulous Paper and then looks at each Smear using the 100X Lens and Immersion Oil. Which of the following Results would you expect to observe? Bacillus Staphylococcus epidermidis E. coli A. Purple Purple Purple _________________________________________________________________________ B. Purple Purple Clear _________________________________________________________________________ C. Purple Purple Pink _________________________________________________________________________ D. Pink Pink Pink
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MIC 101 Exam 1 Page 10
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