Containment level 2 all the requirements of cl1 plus

  • No School
  • AA 1
  • 56

This preview shows page 15 - 25 out of 56 pages.

Containment Level 2 - all the requirements of CL1 plus Facilities • Restricted access • Dedicated hand wash basin at exit • Adequate space for safe working • Separate storage area for lab coats in lab • Negative pressure if mechanically ventilated * Microbiological safety cabinet may be required if infectious aerosol produced
Image of page 15

Subscribe to view the full document.

Containment Level 2 Working practices • Same basic working practices but applied to a higher standard • Greater level of information, instruction, training and supervision required – workers to receive training in handling pathogens Extras depending on nature of work and route of infection Avoid using sharps Gloves • Microbiological safety cabinet • Designated work station • Eye protection • Vaccination
Image of page 16
Containment Level 3 all the requirements of CL2 plus Facilities • Operate at negative pressure • HEPA filter on extract • Sealable for fumigation • Separated from other activities • Viewing panel • Self contained facility • Microbiological safety cabinet (ducted)
Image of page 17

Subscribe to view the full document.

Containment Level 3 Working practices • High standard of training and supervision • All working practices rigorously controlled, documented and recorded • Use dedicated lab coats and autoclave before laundering • When handling infectious materials wear gloves and carry out all manipulations in safety cabinet • No sharps
Image of page 18
Containment Level 4 Totally isolated Complete change of clothes Shower out Sterilise all waste and effluent Double ended autoclave Very strict air handling arrangements
Image of page 19

Subscribe to view the full document.

Assessing risk for work with blood and human tissues Hazards – blood borne viruses – hepatitis viruses, HIV – other pathogens (SARS etc.) Vary depending on – type of material – source of material Risk assessment must also take nature of work into account
Image of page 20
Control measures for work with blood and human tissues Containment Level 2 • Gloves • Avoid use of glass and sharps • Designated work area
Image of page 21

Subscribe to view the full document.

Biosafety Levels BSL 1 : agents not known to cause disease BSL 2 : agents associated with human disease BSL 3 : indigenous/exotic agents associated with human disease and with potential for aerosol transmission BSL 4 : dangerous/exotic agents of life, threatening nature
Image of page 22
Criteria used for biosafety level Source and nature of introduced DNA Recipient organism Nature of expressed proteins Local environment Experimental procedures The biosafety conditions ensures the maximum protection while dealing with any potential hazardous organisms used in different aspects of biotechnology. So that greater protection can be planned for the expected hazard
Image of page 23

Subscribe to view the full document.

Hazards of biological materials Infection Toxic Allergenic Carcinogenic Other ?
Image of page 24
Image of page 25
  • Fall '19

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Ask Expert Tutors You can ask 0 bonus questions You can ask 0 questions (0 expire soon) You can ask 0 questions (will expire )
Answers in as fast as 15 minutes