Whats expression cloning whats iptg used for fig 6 28

12) What’s expression cloning? What’s IPTG used for (Fig. 6-28)?
13) RNAi (Fig. 6-42), the functions of DICER and RISC. Who are Andy Fire and Craig
Mello?
Why is RNAi such a powerful tool in cell biology?
14) CRISPR (Fig. 6-43). What does the guide RNA do? What does CAS9 do? NHEJ versus
Homology-directed repair. Do you think CRISPR could someday be a therapy for
HIV pro- viruses?

- CAS9: nuclease that cleaves dsDNA, cutting the chromosome
- NHEJ can produce insertion and/or deletion mutations of variable length. HDR mediated
repair can introduce precise point mutations or insertions from a ss or dsDNA template
Chapter 8: Genes, Genomics, and Chromosomes
1) What is a gene? Define it as we did in lecture (on the slides).
3) Eukaryotic Transcription units (Fig. 8-3). What is an intron and what's alternative
splicing (Fig. 8-3)?
4) A fundamental difference between the yeast genome and the mammalian genome (Fig. 8-
4).
What's a pseudogene? (e.g. Fig. 6-4a) What's the C value paradox? What explains
the paradox?