The terms aseptic technique and sterile technique are

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Containers and without letting our Bacteria out of these Containers. The Terms “Aseptic Technique” and “Sterile Technique” are informally used interchangeably by most Microbiologists and Cell Biologists. However, the two Terms have subtly different Meanings: Aseptic Technique involves working with only the Organisms of Interest. We ʼ ll be using Aseptic Technique to transfer E. coli from one Petri Plate to another later on in Lab. Sterile Technique involves working without any Life Forms. We ʼ ll be using Sterile Technique later on in the Term when we add Sterile Media to Sterile Test Tubes. Your Mouth, Skin and Hair are major Sources of Contamination. Don’t talk, sing or whistle while performing Aseptic Technique or Sterile Technique. Never touch the Contents of any Culture (this is for your own Safety as well as for Sterility) and don’t lean over the Test Tubes or Petri Plates while you’re working. ___________________________________________________________ “Han Solo. I'm captain of the Millennium Falcon. Chewie here tells me you're lookin' for passage to the Alderaan system?” Yes indeed, if it's a fast ship.’ Fast ship? You've never heard of the Millennium Falcon? “ Should I have?’ It's the ship that made the Kessel Run in less than twelve parsecs. I've outrun Imperial starships. Not the local bulk cruisers mind you, I'm talking about the big Corellian ships now. She's fast enough for you old man. What's the cargo?” Only passengers. Myself, the boy, two droids... and no questions asked.’ What is it? Some kind of local trouble?” Let's just say we'd like to avoid any Imperial entanglements.’ © 1977 LucasFilm Ltd.
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Lab 1 Page 20 Plate Media (Atlas Section 2) There are several different Types of Agar we’ll be routinely using in MIC 101. Some are identifiable by their unique Color; others are marked with a colored Strip on the Side. Nutrient Agar (Black Stripe) Nutrient Agar, our default Medium, contains a bit of Beef Extract. Just about anything will grow reasonably well on Nutrient Agar. It’s a translucent Cream Color. Yeast, Glucose, Calcium Carbonate Agar / YGC Agar (Red Stripe) YGC Agar is the Medium that we use for Micrococcus luteus , Staphylococcus epidermidis and Streptococcus lactis . It’s opaque because the Calcium Carbonate won’t go into Solution unless the Medium is Acidic. When Staphylococcus epidermidis or Streptococcus lactis are grown on YGC, they acidify the Medium around each Colony, producing a characteristic Zone of Clearing. Micrococcus leteus does not acitidy the Medium and so is not surrounded by a Zone of Clearing. Sodium Benzoate Agar (Green Stripe) Sodium Benzoate Agar is used to select for Pseudomonas sp., which happens to be one of the few Genera that can utilize this artificial Compound as a Carbon Source. Fluorescence Agar / F Agar (Brown Stripe) F Agar is a low Iron Medium used to identify Pseudomonas sp. When Pseudomonas sp. are grown on F Agar they secrete characteristic fluorescent Compounds (Siderophores) in an Attempt to gather-in Iron.
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