A. What are P-granules and polar granule complexes comprised of? B. Name three roles of these complexes in the germ cells. 1. ____________________________________ 2. ____________________________________ 3. ____________________________________ Interestingly, a single-stranded RNA binding protein known as dead endwas found to localize to germ plasm throughout all stages of PGC development. C. In what anatomical structureof the gonads of male mouseembryos do you expect to find dead endmRNA? Assume the gonad is no longer ‘indifferent’. ____________________________________Primordial germ cells (PGCs) must migrate within the vertebrate embryo to reach their final destination. The Cxcr4gene encodes a chemokine receptor required for PGC migration in zebrafish and mammals, and the ligand for this receptor is known as SDF-1. D. If PGCs express Cxcr4, in what tissue/structure(s) would you expect SDF-1 to be expressed in mouse embryos? Briefly explain/justify your response.E. How could you show that SDF-1 is sufficientfor the migration of PGCs? Describe an experiment that would show this in a zebrafish. Clearly state the expected outcomeif SDF-1 is sufficient.
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