Fish probe timing of replication correlates with

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FISH probe
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Timing of replication correlates with nuclear location: expressed genes generally replicate early Swedlow and Lamond (2001) Genome Biology 2, 2.1-2.7 Block cells at G1/S with aphidico Release and pulse label with Brd Immunofluorescence labelling o halogenated DNA
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Chromatin : Structure package DNA AND A Regulatory Role open and closed states
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Two functions of histone modifications: (1) structure/stability of nucleosome, (2) binding of other regulators
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from Robertson and Wolffe (2000) Nat Rev Genetics 1, 11-19 A transcriptionally active region targeted for silencing is proposed to acquire DNA methylation first, which then recruits the methyl-CpG binding proteins and their associated co-repressors and histone deacetylases (HDACs). As DNA methyltransferase 1 (DNMT1) can interact directly with histone deacetylase, it is also possible that transcription is first silenced by deacetylation by other tethering factors, after which the methylation machinery and the methyl-CpG binding proteins are recruited to 'cement'the promoter in the silent state. In either case, the deacetylated nucleosomes adopt a more tightly packed structure that inhibits the access of transcription factors to their binding sites.
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* ** Bannister & Kouzarides Cell Research Vol 21 No 3 | March 2011
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Chromocentres: densely-packed heterochromatin, marked with H3K9Me3 and H4K20Me3 from Gunnar Schotta, Ludwig-Maximillians U FISH probe
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