Purple staining gram positive cells pink staining

Info icon This preview shows pages 5–7. Sign up to view the full content.

- purple staining, Gram-positive cells - pink staining, Gram-negative cells These two types of cells differ significantly in the chemical and physical structure of their cell wall. The structure of the thinner cell walls of Gram negative bacteria cannot hold the dyes previously used, once the decolorizer is applied. + u - G To prepare Gram bacterial smear for staining next week: Draw three circles on slide using wax pen. Also include a “G” to identify that slide will be Gram stained. Flip slide over. Use DI water dropper to place very small drop of water inside each circle. Using a sterilized inoculation loop , take a small sample of your unknown. Be gentle! The bacteria is on the surface of the medium. Swirl into the water in the center circle of your slide. Q: Why are there two additional circles on our slide? Use same method to add controls to circle on left and right. Heat fix the slide on top of your microincinerator . Allow it to stay in the platform for 5 minutes after water has completely evaporated. From the Virtual Microbiology Classroom on Watch video of How to Prepare a Bacterial Smear for Gram Staining
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

Acid-fast Stain Distinguishes cells that have mycolic acid in cell wall, from those that do not: - purple staining, Nonacid-fast cells (NAF) - bright pink staining, Acid-fast cells (AF) Cell are considered to be Acid-fast if they have mycolic acid in their cell wall. Q: What is mycolic acid, and which types of bacteria have it? + u - A To prepare Acid-fast bacterial smear for staining next week: Draw three circles on slide using wax pen.
Image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern