Inactivating lac repressor a mutation where lac

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inactivating lac repressor; a mutation where lac repressor no longer binds lactose). (12 pts) Growth conditions WT Mutant A Mutant B Mutant C glucose low; lactose low 2 1 95 2 glucose high; lactose low 1 1 10 1 glucose high; lactose high 8 9 9 1 glucose low; lactose high 100 10 98 2 Mutant A Mutant B Mutant C
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Biology 172 Third Exam 15 MPF activity Time Time Time Time A B C D MPF activity MPF activity MPF activity 42) The graph in panel A illustrates the change in MPF (also called M-Cdk) activity from the end of S phase (marked by the arrows) through the G2 to M phase transition in growing cells. Please indicate how the MPF activity graph would change in the following three abnormal conditions. (9 pts) B) A mutant lacking the Wee 1 kinase. C) A mutant lacking the Cdc25 phosphatase D) A mutant cyclin protease that cannot be phosphorylated by MPF The figure is provided to remind you of the role of Wee1 and Cdc25.
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Biology 172 Third Exam 16 pTCF Amp R gene NotI BclI ApaI ATG TAA EcoRI BamHI BamHI KpnI NotI BclI placO Amp R gene Lac promoter/operator Transcription termination signal MCS BclI EcoRI ApaI NotI 43) You have a plasmid (pTCF) that contains a cDNA for the TCF gene. You wish to express TCF protein in E. coli using the placO plasmid. Briefly outline (step by step) how you would clone the TCF cDNA into the placO plasmid so you can express it in E. coli . MCS = multiple cloning site. The triangles represent stretches of DNA with a high density of restriction endonuclease recognition sites. (12pts) EcoRI G AATTC CTTAA G BamHI G GATCC CCTAG G ApaI GGGCC C C CCGGG NotI GC GGCCGC CGCCGG CG KpnI GGTAC C C CATGG BclI T GATCA ACTAG T
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