According to the lab module which 5 wells of the api

1. According to the lab module, which 5 wells of the API strip should be layered with mineral oil?

2. How many milliliters of water were added to the tray prior to incubating the strip at 37C overnight? 3. What is the main advantage of using the API test? 4. Enter the API Code for S1 and S2 from the lab here:
LAB 8
1. The word ‘antigen’ is actually a combination for what two words?
2. For the ELISA assay depicted below, what do the arrows indicate? 3. What would happen in terms of the colorimetric readout if you forgot to do the wash step after
Step D (above)?

1. In a Western blot ____________ are separated based on its size. 2. When setting up a western blot, what is the purpose of the blocking step post-transfer? 3. When developing a western blot what two factors influence the intensity of the band? 4. Identify (specific name) given to the region of the western blot indicated by the arrow.
1. What is the common name for agglutination?

2. In the wet lab portion of the video, what pathogen was the agglutination assay specifically
designed to test for? Choose all that apply.
3. What was the purpose of the first quadrant of the agglutination assay containing only the
reagent?
4. Based on the image below, which row (A or B) is negative for agglutination?
5. A patient with type O blood is sent to the lab for a blood-clotting assessment. Since type O blood
contains no antigens, which quadrant (#1-6) best depicts the result you would expect to see once
the assay is complete?

LAB 9
Using the observations made and recorded from PL09 Unknown Pathogen, complete the
results sections accordingly.