Treatment of larvae with
F. oxysporum
led to increase in
apoLp-III level in the hemolymph and hemocytes, oppo-
site to the level in fat body.
In the non-denaturating electrophoresis immunoblots of
challenged larvae hemolymph additional protein band ap-
peared. This band was stronger both 15 and 60 min after
immunization, suggesting that during immune response
apoLp-III forms complexes with some molecules.
All of these results suggest that type of pathogen and ex-
position time on it, affect distribution of apoLp-III in in-
sect body.
Because of the fact that there are significant similarities
between the role of mammalian apolipoproteins and in-
sect apolipophorins in innate immune response, we hope
our results obtained on the insect model will contribute to
broadening the knowledge about functioning of apoE in
mammalian immunity.
Acknowledgements
The work was supported by grant N N303 580239 from the Polish Minis-
try of Science and Higher Education.
P2.77
Comparison of methods filtering signal
from magnetic resonance spectroscopy
Michał Staniszewski, Andrzej Polański
Faculty of Automatic, Electronics, and Informatics, Silesian University of
Technology, Gliwice, Poland
e-mail: Michał Staniszewski <[email protected]>
Nuclear magnetic resonance is used as a physical back-
ground for magnetic resonance spectroscopy (MRS) and
magnetic resonance imaging (MRI). Both methods are cur-
rently useful in medicine to investigate a number of hu-
man's diseases including cancer in brain, breast and prostate.
While MRI can give specific knowledge about localization
of investigated tissue, MRS on the other hand gives scien-
tists biochemical information about researched tissues and
tells how aggresive tumour can be. Signal detected in MRS
is exposed to radio frequency pulse and transformed into
a spectrum. Several impovements are necessary in order to
use results of MRS as diagnostic tool. Few limitations of
measurement such as low concentration of metabolites and
limited time of observation can cause low signal to noise
coefficient. However, after measurement methods called
pre-processing techniques are used for enhancement. One
of the pre-processing technique depends on noise which is
present after measurement. Currently there are many meth-
ods which lead to filter signal including implementation of
time domain filters or basing on correctly identified model,
namely Cadzow method. Presentation consists of descrip-
tion of filtering methods and comparison of results.
Acknowledgements
The work was supported by "DoktoRIS - Program stypendialny na rzecz
innowacyjnego Śląska".
Session 2. Genomics, Proteomics, Metabolomics — Medical and
Environmental Implications
69
The First Polish-German Biochemical Societies Joint Meeting, 2012
P2.78
Nucleic acid chaperone properties
of the HIV-2 nucleocapsid protein
Katarzyna Pachulska-Wieczorek, Agnieszka K. Stefaniak,
Katarzyna J. Purzycka, Ryszard W. Adamiak
Institute of Bioorganic Chemistry Polish Academy of Sciences,
Laboratory of Structural Chemistry of Nucleic Acids, Poznań, Poland
e-mail: Agnieszka Stefaniak <[email protected]>
