100%(2)2 out of 2 people found this document helpful
This preview shows page 50 - 52 out of 59 pages.
Treatment of larvae with F. oxysporumled to increase in apoLp-III level in the hemolymph and hemocytes, oppo-site to the level in fat body.In the non-denaturating electrophoresis immunoblots of challenged larvae hemolymph additional protein band ap-peared. This band was stronger both 15 and 60 min after immunization, suggesting that during immune response apoLp-III forms complexes with some molecules.All of these results suggest that type of pathogen and ex-position time on it, affect distribution of apoLp-III in in-sect body.Because of the fact that there are significant similarities between the role of mammalian apolipoproteins and in-sect apolipophorins in innate immune response, we hope our results obtained on the insect model will contribute to broadening the knowledge about functioning of apoE in mammalian immunity.AcknowledgementsThe work was supported by grant N N303 580239 from the Polish Minis-try of Science and Higher Education.P2.77Comparison of methods filtering signal from magnetic resonance spectroscopyMichał Staniszewski, Andrzej PolańskiFaculty of Automatic, Electronics, and Informatics, Silesian University of Technology, Gliwice, Polande-mail: Michał Staniszewski <[email protected]>Nuclear magnetic resonance is used as a physical back-ground for magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI). Both methods are cur-rently useful in medicine to investigate a number of hu-man's diseases including cancer in brain, breast and prostate. While MRI can give specific knowledge about localization of investigated tissue, MRS on the other hand gives scien-tists biochemical information about researched tissues and tells how aggresive tumour can be. Signal detected in MRS is exposed to radio frequency pulse and transformed into a spectrum. Several impovements are necessary in order to use results of MRS as diagnostic tool. Few limitations of measurement such as low concentration of metabolites and limited time of observation can cause low signal to noise coefficient. However, after measurement methods called pre-processing techniques are used for enhancement. One of the pre-processing technique depends on noise which is present after measurement. Currently there are many meth-ods which lead to filter signal including implementation of time domain filters or basing on correctly identified model, namely Cadzow method. Presentation consists of descrip-tion of filtering methods and comparison of results.AcknowledgementsThe work was supported by "DoktoRIS - Program stypendialny na rzecz innowacyjnego Śląska".
Session 2. Genomics, Proteomics, Metabolomics — Medical and Environmental Implications 69The First Polish-German Biochemical Societies Joint Meeting, 2012P2.78Nucleic acid chaperone properties of the HIV-2 nucleocapsid protein Katarzyna Pachulska-Wieczorek, Agnieszka K. Stefaniak, Katarzyna J. Purzycka, Ryszard W. AdamiakInstitute of Bioorganic Chemistry Polish Academy of Sciences, Laboratory of Structural Chemistry of Nucleic Acids, Poznań, Polande-mail: Agnieszka Stefaniak <[email protected]>