The antibiotic sensitivity test of the prepared herbal hand sanitizer against

The antibiotic sensitivity test of the prepared

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extracts of Ocimum sanctum, Eugenia caryophyllus and Cymbopogon flexuous. The antibiotic sensitivity test of the prepared herbal hand sanitizer against skin pathogens was checked using disc diffusion method and results were compared with commercially available synthetic hand sanitizer. Results depicted that the herbal hand sanitizer gives larger inhibition zone than the commercially available synthetic hand sanitizer against Staphylococcus aureus and Pseudomonas aeruginosa. The efficacy of herbal hand sanitizer was evaluated using microorganism suspensions; which revealed that the herbal hand sanitizer is efficient in reducing higher number of microorganism from the hands as compared to commercial synthetic hand sanitizer. Thus, owing to higher antimicrobial activity and efficacy these herbal extracts can be used in the preparation of herbal hand sanitizers on commercial scale. Agnihotri S, Wakode S, 2010, in a study entitled ”Antimicrobial activity of essential oil and various extracts of fruits of greater cardamom conducted byAgnihotri S and Wakode S, Greater cardamom (AmomumsubulatumRoxb. Zingiberaceae) commonly known as "Bari ilaichi" is a well-known plant used in Ayurvedic and Unani medicine. Cardamom has been used for the treatment of various diseases and disorders like gastric ulcer. Therefore antimicrobial activity of petroleum ether, methanol and aqueous extracts from leaves and roots, essential oil and isolated vasicine from A. vasica were tested against various microorganisms. Antimicrobial activity was done by disc diffusion method. The 17
zone of inhibition observed was compared with that of standard drugs, ciprofloxacin and fluconazole. Minimum inhibitory concentration was determined against microorganisms used in the study. The results of this study reveal that methanol extract of fruits of A. subulatum shows remarkable antimicrobial activity against Escherichia coli whereas in case of other microorganisms used the extract was found inferior to the standard drug used. Methanol extract of rind showed good antimicrobial activity against Staphylococcus aureus. The essential oil isolated was found effective against majority of microorganisms used viz. Bacillus pumilus, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Saccharomyces cerevisiae. In a study entitled ”Polyherbal Hand sanitizer - Formulation and Evaluation” conducted byFatima Grace, Sowmya , DarsikaC.,ArulJothy, S. Shanmuganathan, The main aim for the preparation of a poly herbal hand sanitizer is for “hand hygiene”. The preperation is a vital principle in the prevention, control, and reduction of any acquired infection. Mainly hand sanitizer can stop the chain of transmission of micro organisms and other bacteria from hand to different parts of our body. Hand hygiene is important and one of the most critical steps in food production, food service as well as in homes and other day care preparations. Hand sanitizer avoids adverse effects like itching, irritation, dermatitis etc. So, maintaining hand hygiene as the prime criteria- instead of some synthetic formulation, an attempt has been made to formulate an

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