Constant regiondepends on antigen it will attach to

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Constant Region—depends on antigen it will attach to and genetics. Has a carboxyl end (COOH). The carboxyl-terminal segment (half of immunoglobulin light chains or ¾ of heavy chains) of antibody molecules that consist of a polypeptide sequence found in all chains of that type. Variable Region—The amino- terminal region (NH4) of an immunoglobulin molecule (1/2 light chain or ¼ heavy chain) that has a unique amino acid sequence for each different immunoglobulin molecule. This part is responsible for the specificity of a particular immunoglobulin molecule. Secretory Piece or component—a protein with a molecular weight of 70,000 that is synthesized in epithelial cells and added to IgA to facilitate transport of IgA to mucosal surfaces. ****Notes**** FAB2 prime—(fab)2’ binds antigen to molecule Light chains have variable and constant region Isotype—animal vs human immunoglobulins (how to tell the difference between the 2) Allotype—not all humans have the same allotype. Found in heavy chains. Variation of DNA which gives variation of amino acids between people and animals (heavy and light chain) Idiotypes—Variation of the variable region for both heavy and light chains 3. List the following properties of the immunoglobulin classes Serum Concentration, physiochemical properties and biological activity. IgM Heavy chain — µ MW —150,000 doultons, 900 kd Valence — 5 functional binding sites Serum Concentration — 120-150
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Composition— (2µ, 2K)5’J, (2µ, 2λ) 5’J Binds Compliment — Yes Cross Placenta —no Function —first antibody molecule that is produced. Takes 1 molecule of IgM to bind compliment. IgG Heavy chain— γ gamma MW—150,000 d (basic structure) Valence— 2 binding sites Serum Concentration—800-1600 mg/dl Composition—(2γ 2K) or (2γ 2λ) Binds Compliment—yes Cross Placenta—yes Function—where fetus gets protection (crosses placenta), antibody that provides most protection for the organism. IgA Heavy chain— α alpha MW—serum 160kd, fluid 400kd Valence—2 binding sites Serum Concentration—70-350 mg/dl Composition—serum basic structure (2 α, 2 λ) or (2 α, 2 K) Fluid—(2α, 2K)2’J * secretory component (SC) ***Secretory Component- spit, tears, mucous, breast milk*** Binds Compliment—no Cross Placenta—no
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Function—found in body fluid IgD Heavy chain— δ delta MW—150,000 (single molecule) OR 150 kd Valence—2 binding sites Serum Concentration—3 mg/dl Composition—(2δ, 2K) or (δ2, 2λ) Binds Compliment—no Cross Placenta—no Function—unknown, maybe cellular immunity??? IgE Heavy chain—ε epsilon MW— 150 kd Valence—2 binding sites Serum Concentration—.002 mg/dl Composition—(2ε, 2K) or (2ε, 2λ) Binds Compliment— NO Cross Placenta—NO Function—antibody responsible for allergies 4. Describe the laboratory test for the quantitation of immunoglobulins.
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