Lining up the na membrane and the acrylamide exactly

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the acrylamide exactly is not possible even if all precautions are taken. Additionally, the migration distance of the dye wasn’t measured on the acrylamide but estimated from the NA membrane. Even with these errors, the R2tells us the data isn’t very random. 5 | P a g e
Discussion Questions1.Choosing between ELISA and Western blot is an issue of the required data. ELISA is best for quantification where the various methods can be cherry-picked depending on the required accuracy. Unfortunately, as was seen by this experiment, cross-contamination between the wells is a real problem. Western blotting does well to isolate the Ag with the added assurance of the molecular weight. The Western technique doesn’t allow for quantification and requires much more time, however. a.Any detection experiment would be best for Western blot. A good example is a pregnancy test. The body produces hCG when the host is pregnant. hCG is the Ag in this experiment, so the Ab would need to be bound to the test strip. When hCG is released in urine or serum, it will bind to the Ab on the test strip. The strip can be washed with the secondary Ab and the enzyme to indicate the presence of the Ag; alerting the user to pregnancy. b.The sensitivity of the ELISA make it a prime candidate for quantifying any type of protein (Ag) from any source as long as there is a possible Ab. The amount of some virus or bacteria present in tap water after flooding is a good example. CitationFigure 1, Campbell, Neil A; Reece, Jane B; Mitchell, Lawrence G. Biology5thed. Menlo Park, CA. Addison Wesley Longman, Inc. 1999. Print. 6 | P a g e

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