comes about by regulating whether a gene is transcribed, whether/how it is spliced, how it traffics inside the cell (such as if it goes to the cytoplasm for exposure to translational machinery or is sent for degradation), whether it is translated, and how it is modified once a protein has been synthesized. 3. What is one potential criticism of your fate map data. One potential criticism is that your data are incomplete/inaccurate due to the nature of the fate mapping procedure. You used a fluorescent dye to perform the fate mapping. Labeling cells by adding a dye has a major caveat: you need to ascertain to what extent the dye might be diluted such that you are unable to detect potential progeny of the first labeled cell(s). 4. From these data can you infer whether the eye progenitor in the early embryo was either specified or determined to become an eye. No, you cannot. The experiment merely tracked the fate of the eye progenitor. A fate map is a reliable prediction of the events that will transpire during development—it never tells us the commitment of a cell at any particular stage. Commitment is only ascertained by discrete experimental tests. To test specification, the eye progenitor would need to be isolated and allowed to develop in a neutral environment; to test determination, the eye progenitor would need to be isolated and placed into another distinct environment in the embryo and tested for whether or not it still formed an eye. 5. What does this experiment suggest about the mechanism of development that is operative with regard to the eye? In your answer, clearly define the difference between mosaic and regulative mechanisms of development.
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