microbiology to be able to successfully grow bacteria in these plates for observation reasons. In another part of the lab simple stain slides were created. One slide was of E.coli and the other one was of S. aureus. Both of the slides were stained with crystal violet making the bacteria under the microscope purple. For the E. coli slide being viewed under 1000x it was observed that they are individual rod shapes and create little clusters. For the S. aureus being observed the bacteria was more of a circle shape in big clusters together. When making the slides if you don’t get the smear thin enough and spread out enough it is hard to view under the microscope because you end up just seeing giant globs of bacteria smashed together and cannot make out the individual ones, which makes it hard to observe. To fix this problem when making
the slides it is important that when smearing the bacteria time is taken to really make sure the clumps are spread out more evenly before drying and staining. Being able to create slides is important because when in microbiology most the time you are dealing with bacteria that is to small to see with the naked eye and need to be able to observe things under a microscope to really see what is being worked with.
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- Spring '08
- Hong-Chuan Lin