microbiology to be able to successfully grow bacteria in these plates for observation
reasons.
In another part of the lab simple stain slides were created. One slide was of
E.coli
and the other one was of
S. aureus.
Both of the slides were stained with crystal
violet making the bacteria under the microscope purple. For the
E. coli
slide being
viewed under 1000x it was observed that they are individual rod shapes and create
little clusters. For the
S. aureus
being observed the bacteria was more of a circle
shape in big clusters together. When making the slides if you don’t get the smear thin
enough and spread out enough it is hard to view under the microscope because you
end up just seeing giant globs of bacteria smashed together and cannot make out the
individual ones, which makes it hard to observe. To fix this problem when making

the slides it is important that when smearing the bacteria time is taken to really
make sure the clumps are spread out more evenly before drying and staining. Being
able to create slides is important because when in microbiology most the time you
are dealing with bacteria that is to small to see with the naked eye and need to be
able to observe things under a microscope to really see what is being worked with.

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- Spring '08
- Hong-Chuan Lin