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Disinfectant all star 17mm table 1 describes the

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Disinfectant (allStar)17mmTable 1 describes the resistant levels of the four bacteria to the antibiotic. The zone ofinhibition was measured, and recorded in the data table, which was interpreted to see whether thebacteria was sensitive, intermediate, or resistant to that particular antibiotic. Table 2 is thedescription the affects of Ultra-violet light at certain time intervals on the four bacteria. Thetimes were 30 seconds, one minute, two minutes, three minutes, and a control which was notexposed to any UV light.ForS. aureus, E. coli, andB. subtilius, too much growth was on theplate to count the number of colonies on it.Pseudomonas aeruginosahad no growth on the plate.Table 3 shows the effects of different chemical agents on a pure agar plate. The zone ofinhibition was measured for this part.Discussion:The purpose of this experiment was to determine why antibiotic sensitivity wasimportant, methods used for antibiotic sensitivity, how to perform and interpret a disk diffusiontest to determine antibiotic sensitivity, the difference between minimum inhibitory concentration(MIC) and minimum bacteriocidal concentration (MBC), how to determine an MIC using a tubedilution test, the effects of common disinfectants on various microorganisms, the effects of UVlight on various microorganisms, what an endospore was and why organisms that can formendospores were of particular concern to health care professionals. It was a success.Antibiotic sensitivity was important to fight bacteria, and causing infection in humans.Testing these antibiotics using a disk dispenser on a Mueller-Hinton plate, the zone of inhibition
was measured using a ruler.Ps. Aeruginosais highly resistant to the antibiotics causing it togrow normally around each disk. According to the data collected, it resistant to the followingantibiotics: Penicillin (P), CRO, GM, OX, PB, VA, and Bactitracin (B).S. aureuswas alsoresistant to many of the antibiotics that were tested on it, butE. coliandB. subtiliswere eithersensitive or intermediate to majority of the antibiotics that was tested on it, but they were alsoresistant to many as well. This data was found in Table 1. If an antibiotic or disinfectant wasused, there was a zone of inhibition around the area of the physical or chemical agent. Kirby-Bauer disk diffusion technique was used for this; disks are dropped on to a Mueller-Hinton plateand then inoculated for 24 hours. If the bacteria are resistant in any way to the antibiotic on thedisk, a zone of inhibition formed. The zone of inhibition was the diameter of no growth aroundthe disk of agent. Depending on the length of the circle, the zone was interpreted in three ways.An interpretation of sensitivity (S) was made if the zone diameter was equal to or greater thanthe standard; an interpretation of resistant (R) was made if the zone diameter was less than orequal to the interpretative standard for resistance; zone diameter which fall between sensitive and

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Term
Spring
Professor
DwayneBoucaud
Tags
Bacteria, Zone, Antibiotic Sensitivity

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