Objectives This objective of this experiment is to learn the different microbial growth requirements. The second objective is to determine which environmental conditions allow for the best microbial growth. I hypothesize that oxygen presence, neutral pH, mesophilic conditions, and isotonic conditions will allow for the best microbial growth. Materials and Methods When determining how temperature affects microbial growth, 5 TGY agar plates and our unknown were used. The unknown was streaked onto all plates. The plates were then labeled as 5C, 25C, 35C, 45C, and 55degrees C. The plates were then incubated in their associated temperature chamber. In determining how pH affects microbial growth, a broth culture was placed into 3 TGY tubes. The first tube had a pH of 5, the second had pH 7, and the third with pH 9. The broth cultures were S. Aureus , A. faecalis , S. cerevisiae , and the unknown. After inoculation the tubes were incubated at 35 C for 48 hours. When determining how osmotic pressure affects microbial growth, a broth culture was placed into 4 different tubes. The broth cultures were S . Aureus , E. Coli, H. Salinarum, and the unknown. The tubes had a 0%, 5%, 10%, and 25% Salt Concentration. After inoculation the bacteria were incubated at 35C for 5 days. The last step was to determine how oxygen affects microbial growth. The broth cultures that were used were M. Luteus , C. Sporogenes , S. Aureus , and the unknown. The 1
Usman Syed Microbiology 4/13/2019 known cultures were inoculated onto 2 TGY plates. The first TGY plate was incubated
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