20141002_StudyQuestions_answered.pdf

Whether there is a hydroxyl rna or hydrogen dna

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whether there is a hydroxyl (RNA) or hydrogen (DNA) residue attached to the 2’ position of the ribose ring). This study resource was shared via CourseHero.com
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7. You wish to sequence the short DNA sequence shown below using the Sanger method. You only have the equipment to do this in the old-fashioned way. You set up four reactions, each of which contains the DNA to be sequenced (template), a radiolabeled primer (radiolabel is indicated by *) that can hybridize to the template, DNA polymerase, and the deoxynucleotides (dNTPs) and dideoxy nucleotide (ddNTP) indicated below. Primer: *5’ ----------3’-OH Template: 3’-----------ACGACGCAGGACATTAGAC-5’ Reactions contain the following nucleotides: A. dATP, dTTP, dCTP, dGTP, ddTTP (results shown below) B. dATP, dTTP, dCTP, dGTP, ddATP C. dTTP, dGTP, dCTP, ddCTP, ddATP D. dATP, dCTP, dTTP, ddGTP The picture below shows the results when reaction A was run on a gel and subjected to autoradiography. Draw in the results of the other reactions. This study resource was shared via CourseHero.com Powered by TCPDF ( )
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