Always re sterilize your loop before returning it to

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Always re-sterilize your Loop before returning it to the Black Block on your Bench Top. Test Tubes We use Non-Disposable Glass Test Tubes with Plastic Caps. Since these Caps are loose, avoid picking-up a Test Tube by its Cap. We generally use large Test Tubes, which are located in a recycled large Stationery Box on the Side Bench by the Water Baths. Later in the Lab we’ll use small Test Tubes, which will be in a small, Cardboard Box next to this large Stationery Box. Please do not write on our Test Tubes or on our Test Tube Caps. The Writing will not be removed by the Dishwasher and it will then be “baked” on in our Autoclave. Test Tubes should be labeled using the colored Lab Tape. Get a Piece of Tape very slightly longer than you think you’ll need. Fold-over the terminal 1/2 Centimeter of this Tape to make a Quick-Release Tab. Lightly adhere the Tape to your Lab Bench and use your Sharpie to make the Label. Then remove the Tape from the Bench -- isn’t that Quick-Release Tab handy? -- and adhere it to the side of the Test Tube. Please remove this Tape before placing the Test Tubes in the Waste Cart. Always carry Test Tubes in a Test Tube Rack or in one of our White Plastic Containers.
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Introduction to MIC 101 Page 7 Serological Pipettes We use Disposable calibrated, sterile 5 ml Serological Pipettes. Our Pipettes are calibrated from the Top to the Bottom: (Top) 0, 1, 2, 3, 4 (Bottom) The “TD” on these Pipettes stands for “to Delivery” and indicates that you need to “blow-out” all of the Fluid in the Pipette for proper Measurement. We use Green Pi-Pumps (aka Lightsabers) for all Pipetting. Our Pipettes are sterilized inside individual Wrapping that separates sorta-kinda like a Band-Aid Wrapper. Don’t remove the Pipette from its Wrapper until right before you use it and return it to its Wrapper when you’re ready to dispose of it in thePlastic Bus Pan on the Waste Cart. Microtiter Pipettes When we need incredibly precise Measurements or rapidly repeated Measurements we’ll use Microtiter Pipettes. These guys are infinitely more Accurate than a Serological Pipette with a Lightsaber and they’re much easier and faster to use (once you get the Hang of using them). They are also very Expensive ($285 each). We use two sizes of Microtiter Pipette in MIC 101: Name Top Button Color Setting (Maximum) Microliters P200 Yellow 2 | 0 | 0 200.0 P1000 Blue 1 | 0 | 0 1,000.0 The Volume is set by turning the knurled, Black Volume Adjustment Knob on the Upper Portion of the Handle (and if it’s knurled [“ribbed”], the White Control Knob on Top of the Microtiter Pipette). The Volume Indicator Display is on the Front of the Handle. The Volume Indicator Display is read from Top to Bottom. All Digits on the P200 Mitcrotiter Pipettes are Black. A Setting of 1 | 5 | 0 indicates a Volume of 150 μ l; a Setting of 2 | 0 | 0 indicates a Volume of 200 μ l The First Digit on the P1000 Microtiter Pipette is Red (and will be a 0 unless set to Maximum Volume of 1000 ml); the other Digits are Black. A Setting of 1 | 0 | 0 indicates a Volume of 1000 μ l; 0 | 2 | 0 indicates a Volume of 200 μ l; 0 | 1 | 5 indicates a Volume of 150 μ l.
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Introduction to MIC 101 Page 8 MIC 101 Lab Clean-Up Procedures The MIC 101 Waste Carts • Used Petri Plates are dumped in the Red Rubbermaid Garbage Can (“Oscar”) for sterilization
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  • Winter '09
  • MANN
  • Bunsen burner, Pipette, lab bench

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