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Don ʼ t overlap with your a section more than once

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Don ʼ t overlap with your “A” Section more than once, or you ʼ ll be Streaking way too many Bacteria into your “B” Section. 14. Re-sterilize your Loop in your Bunsen Burner. 15. Cool your Loop by touching the Edge of the Agar in an unused Part of your Plate. Again, the Middle of the “T” works well. 16. Rotate your Plate by 90 Degrees (again) such that you can glide your Loop through the ”B” Section once and then streak in a Zig-Zag across the other Side of the Base of the “T”, or roughly the remaining 1/3 of your Plate (which we call the “C” Section). Don ʼ t overlap with your “B” Section more than once. 17. Place the Bottom of your Petri Plate back on its Lid. 18. Remember to sterilize your Loop a final Time before setting it back in its Wooden Holder. This is one of the more Common Mistakes made in Lab. You ʼ re all finished doing your Stuff, but you forget that the Loop still has Bacteria on it that are incredibly Alive. Please do not ever lay your Inoculating Loop on the Benchtop. Repeat the Streaking procedure with your other Plates. Tape your Clan ʼ s 4 Inoculated “Skin” YGC Plates (Red Stripe) together and place this Stack of 4 Plates in the 37°C 48 Hours Boarding Group (Orange Tape). Tape your Clan ʼ s 4 Inoculated “Rectal” Nutrient Agar Plates (Black Stripe) together and place this Stack of 4 Plates in the 37°C 24 Hours Boarding Group (Orange Tape). Tape your Clan ʼ s 4 Inoculated “Rectal” EMB Plates (Burgundy) together and place this Stack of 4 Plates in the 37°C 24 Hours Boarding Group (Orange Tape). _________________________________________________________________ And now, your highness, we will discuss the location of your hidden rebel base... © 1977 LucasFilm Ltd.
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Lab 1 Page 9 Streaking a Plate (Reader ʼ s Digest Condensed Version): Procedure: 1. Make a “T” on the Bottom of your Petri Plates and label each Plate appropriately. 2. Sterilize your Loop. 3. Cool your Loop in an Empty Area of the Mother Plate 4. Touch the Edge of an Isolated Colony with your Loop. 5. Streak these Bacteria onto the “A” Section of your Plate. 6. Re-sterilize your Loop. 7. Streak through your “A” Section once and proceed to streak your “B” Section 8. Re-sterilize your Loop. 9. Streak through your “B” Section once and proceed to streak your “C” Section. 10. Replace your Petri Plate’s Lid and invert the Plate. 11. Re-sterilize your Loop and return it to its Holder. ___________________________________________________________ “What of the Rebellion? If the Rebels have obtained a complete technical reading of this station, it is possible, however unlikely, they might find a weakness and exploit it.” ‘The plans you refer to will soon be back in our hands.’ Any attack made by the Rebels against this station would be a useless gesture, no matter what technical data they have obtained. This station is now the ultimate power in the universe! I suggest we use it!
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