Crossing over causes the f plasmid to integrate into

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crossing over causes the F plasmid to integrate into the bacterial chromosome A single crossover event leads to integration into the bacterial genome F is an episome , a plasmid that can integrate into the host genome Hfr = High frequency recombination bacterial chromosomes have several IS elements, and 20-30 possible Hfr chromosomes can form
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Transfer of the Hfr chromosomes is similar to transferring the F factor merozygote - partial diploid The whole chromosome is not transferred as conjugation does not last long enough Homologous recombination can occur in a merozygote and therefore transfer genes between bacterial chromosomes Recombination has to occur before degradation of the linear DNA
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Transfer of the Hfr chromosome can introduce new alleles into the recipient Only a portion of the F plasmid is transferred so the recipient is not F + Recombination converted the thr- leu+ strR cell into thr+ leu+ strR that can grow on minimal media supplemented with streptomycin making media with a selective agent (usually an antibiotic) is a popular way to target only the bacterial growth that you are interested in
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Interrupted mating can produce genetic maps The Hfr chromosome is transferred, and genes farther away from the 5 oriT transfer later than genes closer to oriT
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Interrupted mating can produce genetic maps The Hfr chromosome is transferred, and genes farther away from the 5 oriT transfer later than genes closer to oriT These cells can grow on media lacking thr and leu Use the thr+ leu+ cells to test for other transferred genes agitate the culture to stop conjugations at specific times
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Interrupted mating can produce genetic maps agitate the culture to stop conjugations at specific times
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Use multiple Hfr strains to determine the gene order over
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