We needed to use Beers Law to be able to calculate the concentration of the

We needed to use beers law to be able to calculate

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wavelength, an absorptivity coefficient, etc. We needed to use Beer’s Law to be able to calculate the concentration of the analyte and the other samples. It allows us to use absorbance and concentration in an equation to find the unknowns. Table One: Bayer Aspirin & 1:50 ASA Solution Absorbencies and Concentration Table Two: Bayer Aspirin Information Brand Advertised % Aspirin Tablet Mass Abs. of Solution Mass of Aspirin % Aspirin Bayer 325 mg 0.399 g 0.367 2.908 86.7%
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d. Our equations and graph show that the Bayer levels are indeed in-between the 4 mL and 6 mL ASA aspirin solution, which is pretty self-explanatory from the procedure and experimentation. e. The concentration of aspirin is easily located because we first diluted the ASA solution to a 1:50 proportion. We were able to easily crush the Bayer tablet (Table 1) and dilute it in the same way we did the ASA. From there, we just took the spectrometer and established the absorbances for all of the samples. That’s how we discovered that the Bayer is where it is in comparison to the ASA. 5. Reflection: a. The amount of aspirin that we determined was less than what the bottle said. Either this can be attributed to experimental error, or the companies are mislabeling their bottles. b. I think that our lack of complete mixture of the aspirin solution, since we couldn’t get the remaining little chunks mixed into the solution. We attributed that to insolubility rules. c. Our equations didn’t match up with any of the results we were looking to get, we aren’t quite sure how our equations didn’t work for the calculations. We did the math anyway, to show that we understand the concepts. 0 0 0 0 0 0 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 f(x) = 618.11x + 0.04 R² = 1 1:50 ASA Solution vs. Aborbance 1:50 ASA Solutions Linear (1:50 ASA Solutions) mL of 1:50 ASA Solution A b s o r b a n c e
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0 0 0 0 0 0 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 f(x) = 618.11x + 0.04 R² = 1 Concentration 1:50 ASA vs. Absorbance Concentration 1:50 ASA Linear (Concentration 1:50 ASA) Absorbance Values C o n c e n t r a t i o n
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  • Spring '14
  • Genetics, ASA solutions, asa solution

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