Mouthwash a test was a white tea extract mouthwash

This preview shows page 2 - 3 out of 4 pages.

Mouthwash A (test) was a white tea extract mouthwash; mouthwash B (placebo control) was a placebo that constituted distilled water, and mouthwash C (positive control) was a commercially available 0.2% chlorhexidine mouthwash (ICPA Health Care Products, Ankleshwar, Gujarat). For mouthwash A, white tea extract powder (Changsha Nutra-Y Biotechnology, Hunan, China) was used. The solubility of the mouthwash was 1 mg/100 ml of water. It was dissolved in distilled water for an effective concentration of 1%. Subjects with good systemic health with at least 24 scorable teeth (not including third molars or crowned teeth) were included in the study. Subjects who consumed a high polyphenolic diet (including green tea or soya diet), wore appliances, or prostheses (fixed or removable) were prescribed antibiotics or other medications in the previous 3 months or had undergone treatment for periodontal problems were excluded from the study. Subjects having diseases that would increase oxidative stress such as diabetics and subjects not cooperating with the study protocol were excluded from the study. Forty-five students, studying in Terna Dental College, Nerul, Navi Mumbai, India, who complied inclusion criteria, were selected. This study was approved by the Ethical Committee of the same institution and was conducted in accordance with the Declaration of Helsinki and principles of good clinical practice. The subjects were randomly assigned into three equal groups Group A, B, and C (15 subjects per group). At baseline, thorough oral prophylaxis was done for all the subjects by the investigator. Plaque was disclosed using Two Tone Plaque Disclosing Agent (Dento Plac, India) to ensure that all deposits had been removed. Subjects were told not to use toothbrushes, toothpaste, or any other interdental cleaning aid or chewing gum for the next 4 days. Instead, subjects were asked to use one of the assigned mouthwash two times a day. At a particular specified time during the day, all subjects were instructed to rinse two times a day for 1 min with 10 mL of the randomly allocated rinse. At each rinsing, 10 mL of the solution was swished around the mouth for about 60 s and then expectorated. On day 5, the subjects were recalled; plaque index (PI; Turesky et al . modification of Quigley PI) was recorded, and subjects were allowed to resume their routine oral hygiene regimens. Data collection The amount of plaque was checked on days 0 and day 5. Adverse effects such as burning sensation, altered taste, and desquamation of gingival epithelium if any were also evaluated on the 5 th day. In vitro study design The white tea extract was tested against three organisms: Porphyromonas gingivalis (Pg), Porphyromonas intermedia (Pi) and Aggregatibacter actinomycetemcomitans (Aa). Media used The brain heart infusion (BHI) agar was used; agar plates were brought to room temperature before use.
Image of page 2
Image of page 3

You've reached the end of your free preview.

Want to read all 4 pages?

  • Fall '19
  • White Teeth, Mouthwash, Green tea, Camellia sinensis, Chlorhexidine, Journal of Indian Society of Periodontology

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Stuck? We have tutors online 24/7 who can help you get unstuck.
A+ icon
Ask Expert Tutors You can ask You can ask You can ask (will expire )
Answers in as fast as 15 minutes
A+ icon
Ask Expert Tutors