Microscopic observation of cell size and Tonicity MATERIALS AND METHOD As

Microscopic observation of cell size and tonicity

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Microscopic observation of cell size and Tonicity MATERIALS AND METHOD As described in the Human Physiology 1 Practical Manual VU, 2019 No changes were applied according to the materials and methods given. RESULTS Experiment of Microscopic observation of cell size and Tonicity under different solutions Solutes 0 MIN BEAKER 90MIN BEAKER 0MIN TUBING 90MIN TUBING Glucose 0 0 + 0 Protein 0 0 + + Sodium chloride 0 + + + Figure 1.3 illustrates horse blood in 0.9% of solution. Figure 1.4 illustrates horse blood in 0.9% and 0.2% of solution Figure 1.5 highlights the horse blood in 0.5% solution Figure 1.2
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Horse blood was used to conduct this experiment by testing it with solutions of different tonicity. In figure 1.3, it indicates the blood under 0.9% solution has given no change to the cells in size and texture, this finalises that this saline solution is isotonic. If a cell is placed in an isotonic solution, that means the amount of stuff inside the cell and outside the cell is equal which therefore concludes that substances in the intracellular and extracellular regions have no purpose of moving around. In figure 1.4 demonstrates the horse blood had a shrivelled texture and overall smaller size as a appose to Figure 1.3. This shows that the solution was hypertonic and therefore states that water would exit the cell due to a higher concentrated gradient which is the solvent. In figure 1.5 it delves into the illustration of horse blood in 0.9% and 0.2% of saline solution. What can be explained is that the solution is hypotonic. This is due to the solution having a higher concentration than the solvent (blood) , therefore the blood has a lower osmotic pressure than the saline solution. The water would go through a selectively permeable membrane from a lower concentration to a higher concentration.
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