This will entail positioning the bottom of the

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This will entail positioning the Bottom of the Meniscus of Sterile Saline on the 0.5 ml Line between “0” and “1”. Do this at Eye-Level. G. Replace the Lid on the Sterile Saline. You want to maintain Sterile Technique. It helps if your Lab Partner removes and replaces all of these Lids (one at a Time). H. Remove the Lid from one of the sterile Dilution Series Test Tubes and dispense 4.5 ml of Sterile Saline into this Test Tube. Keep your Fingers away from the sterile Working-End of the Serological Pipette. I. Replace the Lid on this Test Tube. Use the same Serological Pipette to add sterile Saline to all six sterile Test Tubes. Return it to its Wrapper when you are through and place this used Serological Pipette -- in its Wrapper-- in the Nalgene Pipette Jar on the Waste Cart.
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Lab 6 Page 6 STOP! You have again landed on a “Take an Oral Quiz” Square Answer me these Questions Three, ere The Ground Beast Sample you ʼ ll see. You and your Clan need to show us a complete Viable Count Experiment Set-Up and provide Semi-Lucid Answers to a Series of Questions about the Viable Count Exercise Protocols (particularly your Spread Plate Strategy). Make your Dilution Series (Aseptic Technique) 1. Your TA will transfer 500 μ l (Set your P1000 at 0 | 5 | 0) of our 10 -1 Hamburger Dilution to your 10 –2 Dilution Tube (2). Vortex your Tube. 2. Using a new Microtiter Pipette Tip, transfer 500 μ l from your 10 –2 Dilution (2) to your 10 –3 Dilution Tube (3). 3. Continue this Procedure until all of your Dilution Tubes through 10 -7 have been set-up. Use a new Microtiter Pipette Tip for each Transfer. STOP! Plan your Spread Plate Strategy • Vortexer arranges, vortexes and holds Dilution Tubes • Pipetter transfers 100 μ l of Dilutions onto Plates. • N-Spreader spreads Nutrient Agar Plates. • MacSpreader spreads MacConkey Agar Plates. If you start at the 10 -7 Dilution Tube (“7”) and work toward the 10 -2 Dilution Tube (“2”), you can use the same Microtiter Pipette Tip for transferring all the Dilutions and the same Spreader on the same Type of Agar (i.e. one sterile Spreader for all Nutrient Agar Plates and one sterile Spreader for all MacConkey Agar Plates). Any Carry-Over from the previous Dilution will be 1/10th that of the next so the Additive Effect will be negligible. If you ʼ re a Clan of Four, proceed to Page 7. If you have a Clan of Three, Spread Wallah replaces both N-Spreader and MacSpreader. Spread Wallah will first do all the Nutrient Agar Plates with one sterile Spreader, then do all the MacConkey Agar Plates with a new sterile Spreader. Advance to Page 8.
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Lab 6 Page 7 Clan of Four Spread Plating 1. Vortexer positions the Dilution Series, Turntable, Nutrient Agar Plates, MacConkey Agar Plates, Pipetter , N-Spreader and MacSpreader for maximum Ergonometric Efficiency.
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