E55-2009 practice exam 2 key

Induces neural tissues by blocking epidermal fate in

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surrounding tissues 3. ‘induces’ neural tissues by blocking epidermal fate in ectoderm 4. Tissue derived from organizer: notochord 24. (12 points) A. Experiment: in situ hybridization or antibody stain; Result: see transcripts or protein in Nieuwkoop center B. Experiment: again, in situ hybridization or antibody stain; Result: see transcripts or protein in location of organizer (dorsal margin, blastopore lip); C. Experiment: microinject gene transcript onto other side of embryo; Result: see duplicate axis; D. Experiment: antisense knockdown, treat animal with antibodies against protein; Result: see loss of dorsal structures and corresponding ventralization of the embryo 25. (4 points) 1. MAYBE 2. While you can deduce that dorsal structures are associated/correlated with inheritance of the gray crescent, this experiment does not distinguish between the gray crescent AND its adjacent regions. The gray crescent is an overt marking of a subregion, but we don’t know for sure if just the gray crescent is the magic juice that makes/signals dorsal structures, though it is a tantalizing marker that segregates with dorsal activity.
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