You would then spread plate 100 μ l from each of

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You would then Spread Plate 100 μ l from each of these Dilutions onto a Nutrient Agar Plate and incubate these Plates at 37°C for 24 Hours. The next Week, you would count the Number of Colonies on the Plates you made at each Timepoint. You would then do the necessary Calculations to determine the Number of Bacteria per Milliliter of Growth Medium in your 37°C Flask in the Shaking Water Bath. Your Data are: Timepoint Colonies E. coli per ml 0 78 7.80 x 10 7 10 94 9.40 x 10 7 20 115 1.15 x 10 8 30 137 1.37 x 10 8 40 166 1.66 x 10 8 50 200 2.00 x 10 8 Semi-Log Graph Paper is on Page 5 of your Exam. What was the Doubling Time for your E. coli in this Experiment? A. 32 Minutes B. 37 Minutes C. 42 Minutes D. 47 Minutes
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MIC 101 Exam 1 Page 5 Semi-Log Graph Paper
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MIC 101 Exam 1 Page 6 7. The First Set-Up for aligning your Microscope involves -- A. • Setting the Working Distance between the Condenser Lens and the Specimen. • You use the Condenser Focus Control, which raises and lowers the Stage without moving the Condenser. B. • Setting the Working Distance between the Condenser Lens and the Specimen. • You use the Condenser Focus Control, which simultaneously raises and lowers both the Stage and the Condenser together as a single Unit. C. • Setting the Working Distance between the Specimen and the Objective Lens. • You use the Fine and Coarse Focus Controls, which raise and lower the Stage without moving the Condenser. D. • Setting the Working Distance between the Specimen and the Objective Lens. • You use the Fine and Coarse Focus Controls, which simultaneously raise and lower both the Stage and the Condenser together as a single Unit. 8. The Second Set-Up for aligning your Microscope involves -- A. • Setting the Working Distance between the Condenser Lens and the Specimen. • You use the Condenser Focus Control, which raises and lowers the Condenser without moving the Stage B. • Setting the Working Distance between the Condenser Lens and the Specimen. • You use the Condenser Focus Control, which simultaneously raises and lowers both the Stage and the Condenser together as a single Unit. C. • Setting the Working Distance between the Specimen and the Objective Lens. • You use the Fine and Coarse Focus Controls, which raise and lower the Stage without moving the Condenser. D. • Setting the Working Distance between the Specimen and the Objective Lens. • You use the Fine and Coarse Focus Controls, which simultaneously raise and lower both the Stage and the Condenser together as a single Unit.
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MIC 101 Exam 1 Page 7 9. The Third Set-Up for aligning your Microscope involves -- A. • Setting the Size of the Cone of Light projected by the Illumination System using the Condenser Diaphragm Control. • Centering this Cone of Light using the Condenser Centering Screws.
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