Stage 0 vegetative cell with 2 chromosomes stage i

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• Stage 0 - Vegetative Cell with 2 Chromosomes • Stage I - Axial Filament Formation • Chromosomes form an elongated Axial Nucleoid • Stage II - Polar Septum Formation • The Cell Membrane of the Spore Mother Cell infolds to segregate the Core Cytoplasm and Nucleoid • Stage III - Engulfment • The Cell Membrane of the Spore Mother Cell engulfs this Septum-encased Core (or Forespore) • Stage IV - Cortex Formation • The Cortex is formed by insertion of Peptidoglycan in-between the two Membranes surrounding the Core • Stage V - Spore Coat Formation • A Keratin-like Spore Coat is produced around the Cortex • Dipicolinic Acid Accumulates • Production of Small Acid-Soluble Spore Proteins (SASPs) • Stage VI - Maturation • Increase in Heat and Chemical Resistance • Endospore becomes Refractile under Phase Contrast • Stage VII - Lysis of the Spore Mother Cell and release of the Endospore
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Growth of Bacteria Page 3 Endospore Formers Aerobic Endospore Formers - Bacillus subtilis Bacillus subtilis is the E. coli of Gram Positives. It is the most studied Gram Positive Bacteirum. It is the Model for Spore Formation. It was one of the first Genomes to be sequenced (4.2 Mbp). - Bacillus cereus Bacillus cereus is our Rogues Gallery Bacillus. It is a very common Bacterium in Soil. A lot of you will end-up isolating this from Experimental Gardens Soil. Bacilus cereus can cause Food Poisoning, particularly in leftover Rice (Steaming Rice doesn ʼ t kill Clostridium cereus Spores). - Bacillus mycoides Bacillus mycoides (G = like a fungus) is a real nuisance in Lab. It has a characteristic “swirly” Appearance (hence its Name) and it can outgrow most of our Lab Cultures. - Bacillus anthracis (Anthrax) Anthrax is a thoroughly Nasty Disease and an all-too-Perfect Agent for Biological Warfare.
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