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L.04.Spores

• the cell membrane of the spore mother cell

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Unformatted text preview: • The Cell Membrane of the Spore Mother Cell engulfs this Septum-encased Core (or Forespore) • Stage IV - Cortex Formation • The Cortex is formed by insertion of Peptidoglycan in-between the two Membranes surrounding the Core • Stage V - Spore Coat Formation • A Keratin-like Spore Coat is produced around the Cortex • Dipicolinic Acid Accumulates • Production of Small Acid-Soluble Spore Proteins (SASPs) • Stage VI - Maturation • Increase in Heat and Chemical Resistance • Endospore becomes Refractile under Phase Contrast • Stage VII Growth of Bacteria Page 2 Spore Formation Spore Formation occurs during the Stationary Phase. The Process takes approximately eight (8) Hours. • Stage 0 - Vegetative Cell with 2 Chromosomes • Stage I - Axial Filament Formation • Chromosomes form an elongated Axial Nucleoid • Stage II - Polar Septum Formation • The Cell Membrane of the Spore Mother Cell infolds to segregate the Core Cytoplasm and Nucleoid • Stage III - Engulfment • The Cell Membrane of the Spore Mother Cell engulfs this Septum-encased Core (or Forespore) • Stage IV - Cortex Formation • The Cortex is formed by insertion of Peptidoglycan in-between the two Membranes surrounding the Core • Stage V - Spore Coat Formation • A Keratin-like Spore Coat is produced around the Cortex • Dipicolinic Acid Accumulates • Production of Small Acid-Soluble Spore Proteins (SASPs) • Stage VI - Maturation • Increase in Heat and Chemical Resistance • Endospore becomes Refractile under Phase Contrast • Stage VII - Lysis of the Spore Mother Cell and release of the Endospore Growth of Bacteria Page 3 Endospore Formers Aerobic Endospore Formers - Bacillus subtilis Bacillus subtilis is the E. coli of Gram Positives. It is the most studied Gram Positive Bacteirum. It is the Model for Spore Formation. It was one of the first Genomes to be sequenced (4.2 Mbp). - Bacillus cereus Bacillus cereus is our Rogues Gallery Bacillus. It is a very common Bacterium in Soil....
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