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Rate of oxygen supply or demand
812.(4pts) You are setting up an experiment to study passive diffusion of a set of solutes across the membrane of epithelial cells. Your lab mate accidentally added a compound to your buffer that killed all your cells. Can you still use the dead cells to study solute flux rates? 13.(4pts) Early this semester you used the hemolysis of erythrocytes to estimate the membrane permeability of different solutes. Despite its relatively low partition coefficient ureahad the fastest hemolysis time. What could account for the rapid hemolysis of the erythrocytes once placed in the urea solution?
914.(6 pts) You set up an experiment to characterize the enzyme “Alanine Aminotransferase” which catalyzes the reversible transfer of an amino group from glutamate to pyruvate to form 2-oxoglutarate and alanine. Your reaction mixtures consists of the following reagents: 1 mM of Alanine Aminotransferase 1 M of glutamate Several concentrations of pyruvate ranging from 1 mM to 1 M. Based on the results you determine the: Vmax=50 mM/minute Km=25 mM/minute For each of the statements below selectthe correct responseand provideshort explanation for your answer. a.You can increase the Vmax of the reaction by increasing the concentration of pyruvate. a