Lecture8_Nucleosome v2

Rate of packing 100bpsec self assembly experiments

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Rate of packing: 100bp/sec Self-assembly
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Experiments Reveal Crystalline Experiments Reveal Crystalline Order for Viral DNA Order for Viral DNA Cryo-electron microscopy reveals that the packaged DNA is highly ordered. Crystalline order characterized by concentric rings of DNA wrapped around a central axis. (Cerritelli et al .)
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Forces and Packing Rates: As a Forces and Packing Rates: As a Function of Fraction Packed Function of Fraction Packed (Bustamante et al .) Force resisting further packaging Rate of packing
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Viral Packing: Relevant Scales Viral Packing: Relevant Scales Capsid size = 40nm (Hendrix) Persistence length of DNA, length over which DNA can be thought of as being stiff. There is a negative charge every .17nm of length along DNA – electrostatic energy crucial also. Note: kT = 4.1 pN nm – coexistence of thermal and deterministic forces Governing dimensionless parameter
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Viral Packing: Free Energy of Viral Packing: Free Energy of Confinement Confinement DNA has elasticity DNA is charged The idea: set up a free energy function that reflects the competition between these two effects (Riemer et al.,Odijk, Gelbart et al.) Hoop model of packed DNA
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