17 1 Non absorbable 2 Must not affect or be affected by the GIT 3 Must mix

17 1 non absorbable 2 must not affect or be affected

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1. Non-absorbable 2. Must not affect or be affected by the GIT 3. Must mix easily with food 4. Easily & accurately measured in samples - Ex. Ferric oxide, chromic oxide, silica, lignan 1. Adapt animal to test diet (which contains a marker) 2. Collect a feed and fecal sample 3. Analyze each for marker and nutrient of interest relative to your indicator Apparent DigestibilityCoefficient = A B A Advantages are that it is less labour intensive, ideal for wild animals Apparent versus True Digestibility Apparent digestibility under-estimates True Digestibility The following are examples of things not considered when calculating Apparent digestibility: - Endogenous secretions o Epithelial cells fatty acids - Bacterial growth in gut o Nutrient synthesis biotin - Digestive enzymes o Protein secretion True Digestibility 1. Perform digestibility study using a TEST DIET 2. Switch to diet containing none of the nutrient of interest (ZERO NUTRIENT DIET). 3. Analyze feces after TEST DIET is cleared 4. Subtract level of nutrient in feces of animals fed the ZERO NUTRIENT DIET from the TEST DIET 18
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True Digestibility True DigestibilityCoefficient = A −( B C ) A A = Rati of Nutrient/Marker I TEST DIET B = Ratio of Nutrient/marker in Feces C = Ratio of Nutrient/Marker in Feces after ZERO NUTRIENT DIET Factors that affect Digestibility Over 50 different factors can influence digestion efficiency These are only a few examples… - Feed intake - Particle size - Chemical composition - Climate - Age 20
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