Tie a knot in the remaining dialysis tubing just above or just below the rubber band. This will create a seal and ensures that solution will not leak out of the tube later in the experiment. 5. To test that no solution can leak out, add a few drops of water to the tubing and look for water leakage. If any water leaks, tighten the rubber band and/or the knot in the tubing. Make sure you pour the water out of the tubing before continuing to the next step.
6. Repeat Steps 4 - 5 with the three remaining dialysis tubes, using each of the three remaining rubber band colors. 7. Reconstitute the sucrose powder according to the instructions provided on the bottle’s label (your kit contains 60 g of sucrose in a chemical bottle). This will create 200 mL of a 30% stock sucrose solution. 8. Use Table 5 to create additional sucrose solutions that are 30%, 15% and 3% concentrated, respectively. Use the graduated cylinder and waste beakers to create these solutions. Set these solutions aside. Table 5: Serial Dilution Instructions Sucrose Solution mL of Stock Sucrose Solution Needed mL of Water Needed 30% 10 0 15% 5 5 3% 1 9 3% 1 9 9. Pour 150 mL of the remaining stock sucrose solution into Beaker 1. 10. Use some of the remaining stock sucrose solution to create an additional 200 mL of a 3% sucrose solution into Beaker 2. Hint: Use your knowledge of serial dilutions to create this final, 3% sucrose solution. 11. Measure and pour 10 mL of the remaining 30% sucrose solution into the dialysis bag with the yellow rubber band. Seal the top of this tubing with the remaining yellow rubber band. 12. Measure and pour 10 mL of the 15% sucrose solution in the bag with the red rubber band, and seal the top of the dialysis tubing with the remaining red rubber band. 10 mL of the 3% sucrose solution in the bag with the blue rubber
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- Fall '19
- 50%, 15 cm, 15 %, 30 %, 3.0 cm