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1.Briefly describe your observation of the moving pigment particles. 2.Describe any changes to the movement of the particles that you see after adding the heated coins. Time Point Measurement Bag A Bag B Bag C Bag D 0 min Initial Weight Change in Weight 0 g 0 g 0 g 0 g 10 min Total Weight Change in Weight 20 min Total Weight Change in Weight 30 min Total Weight Change in Weight 40 min Total Weight Change in Weight 50 min Total Weight Change in Weight
10 Hemolysis TABLE 1HEMOLYSIS OF RED BLOOD CELLS EXPOSED TO THREE SOLUTIONS WITH DIFFERENT SOLUTE CONCENTRATIONSTube Contents Readable Print (yes/no) Microscopic Appearance (drawing) Cell Condition (normal/crenation/lysed) 1 5 mL 10 % NaCl 2 5 mL 0.9 % NaCl 3 5 mL distilled water Plasmolysis Draw your observations below of plant cells in water and in 30% NaCl (include total magnification next to each drawing). Water 30% NaCl
11 Conclusions Osmosis 1.State the tonicity of each bag (A, B, C, and D) relative to the beaker (hypertonic, isotonic, or hypotonic). 2.A concentration gradient for water must be present in cells for osmosis to occur. Which bag(s) represented the steepest concentration gradient relative to its surrounding environment? 3.Refer to your graph. How does the slope of a segment of a curve relate to the rateof diffusion? 4.What influence of diffusion (temperature, pressure, concentration) causes the curves for bags C and D eventually to become horizontal (have a slope = 0)? Briefly explain5.Based upon the sucrose bag exercise, design an experiment to determine solute concentration in potatoes. .
12 Diffusion 6.From what you know of the indicators, which molecules are capable of moving through the dialysis tubing? Justify your answer. 7.Why must particles be extremely small to demonstrate Brownian movement? 8.Through which test tubes could you read the printed page? Why? 9.Which concentration of NaCl lysed the cells? 10.Which of the three solutions most closely approximates the solute concentration in a red blood cell? How do you know?
13 Plasmolysis 11.Why did the plant cells plasmolyze when immersed in a hypertonic solution? 12.What can you conclude about the permeability of the cell membrane and vacuolar membrane to water? 13.Why did the cells not lyse when put in water (a hypotonic solution)?