E55-2010 exam 1 key

Arrayed with the microtubules of the axoneme and

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arrayed with the microtubules of the axoneme, and dynein acts as an ATPase to hydrolyze ATP and thereby change its conformation to trigger movement of the microtubules to generate sperm tail locomotion. 24. Scenario 1: NO; without jelly there is no way to attract sperm or to activate directional movement; also accepted: without jelly, there can be no acrosomal reaction (and no bindin exposed to bind VE). Scenario 2: NO; mammalian sperm must undergo capacitation, a maturation process involving chemical modification of sperms. 25. A. The mechanism of liver development is mosaic, as key component(s) are localized to the p urple cytoplasm. B. Nothing! The mechanism could be entirely mosaic, since we know that the liver organogenesis is mosaic; however, the majority of metazoans use a combination of mosaic and regulative development. Additional data is needed to suggest the overall mechanism of development for the michifish. C. determinant; ‘necessary’ also accepted. D. Add the purple cytoplasm to a location where no liver forms, and see if host forms 2 livers. E. mRNA, protein. 26. A. The gene is pleiotropic and has multiple functions. B. in situ hybridization, RT- PCR, immunohistochemistry. C. conditional knockout. D. DNA constructs: (1) blood specific promoter driving Cre, (2) gene to target with targeting region flanked by loxP sites; Only when blood promoter is active will the excision occur, thus only blood cells will have the gene activity interfered with during development.
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