Amylovora strains from p communis was 99 the

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E. amylovora strains from P. communis was 99%. The difference of this strain was also pointed out by pathogenicity tests and by assessing and comparing genes involved in the pathogenicity of E. amylovora such as hrpN and dspA/E (Giorgi and Scortichini 2005). By using AFLP, Rico et al. (2004) also found a deviating strain isolated from Crataegus sp. in the USA that showed around 62% of dissimilarity with other E. amylovora strains from Maloideae. This confirms once more that a greater genetic diversity of E. amylovora has to be expected from strains isolated in North America, the centre of origin of the pathogen (McManus and Jones 1995a). The assessment of more E. amylovora strains obtained from wild and ornamental Rosaceous plants using differ- ent techniques (PCR-based molecular fingerprinting, pres- ence and characterization of the pEA29 plasmid, gene sequencing) may improve the detection of the pathogen in asymptomatic plants. In fact, nowadays the detection of the pathogen is mainly based on the features shown by the strains obtained from cultivated crops. The creation of an international databank concerning E. amylovora fea- tures would greatly help the detection tasks. By using rep-PCR, similar results were obtained by McManus and Jones (1995a). Also in their study all the strains from Rubus spp. (Rosoideae) clustered separately from the strains from Maloideae, even if ARDRA analysis carried out with Hae III did not reveal any diversity in the 1 2 3 4 5 6 M 517 396 298 c Figure 3 Amplified ribosomal DNA restriction analysis banding pat- terns for amplified 16S rDNA obtained by using Hae III restriction endonuclease for Erwinia amylovora , Erwinia pyrifoliae and Brenneria rubrifaciens strains. M, molecular size marker (1-kb DNA ladder; Gibco-BRL). Lane 1, E. pyrifoliae CFBP 4172; lane 2, B. rubrifaciens NCPPB 2020; lane 3, E. amylovora PD 2915; lane 4, E. amylovora UniCt Sic 1; lane 5, E. amylovora IVIA 1951-2; lane 6, E. amylovora IVIA 1767-3; C, negative control. Similarity 1·00 0·85 E. amylovora ( Maloideae ) PD 103 ( Rubus sp.) PD 2915 ( Amelanchier sp.) NCPPB 2292 ( Rubus sp.) NCPPB 2293 ( Rubus sp.) 0·70 Figure 2 Dendrogram of genetic relatedness of the combined repetitive-sequence PCR data inferred using Repetitive Extragenic Palindromic and Enterobacterial Repetitive Intergenic Consensus primer sets and generated by 93 Erwinia amylovora strains. Unweighted pair group method with arithmetic mean analysis was performed using Dice’s coefficients. The scale indicates the degree of genetic relatedness between strains. From the 93 E. amylovora strains tested, 89 showed the same pattern. Four strains showed distinct banding patterns. D. Barionovi et al. E. amylovora characterization ª 2006 The Authors Journal compilation ª 2006 The Society for Applied Microbiology, Journal of Applied Microbiology 100 (2006) 1084–1094 1091
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restriction pattern between the two subgroups. In the pre- sent work, the three strains from Rubus spp. assessed clus- tered differently upon UPGMA analysis. It is worth noting that by using REP primer any difference between NCPPB 2292 and NCPPB 2293 was observed. Conversely, the util-
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  • Spring '08
  • Devartanian
  • DNA, DNA sequencing, Restriction enzyme, Greece Greece Greece Greece Greece Greece Greece Greece Hungary Hungary Hungary Hungary Hungary Hungary Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy USA USA Italy Italy Italy Italy, E. amylovora

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