What does the pH measure?1 Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile salts, pH 2.0 buffer)?2
Experiment Data Tube No Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time (min) Temp (°C) pH 1 Lipase Vegetable Oil Bile salts pH 7.0 Buffer 60 37 6.21 2 Lipase Vegetable Oil Deionized Water pH 7.0 Buffer 60 37 7.00 3 Lipase Deionized Water Bile salts pH 9.0 Buffer 60 37 9.00 4 Deionized Water Vegetable Oil Bile salts pH 7.0 Buffer 60 37 7.00 5 pH 2.0 Buffer Lipase Vegetable Oil Bile salts 60 37 2.00 6 Lipase Vegetable Oil Bile salts pH 9.0 Buffer 60 37 9.00 Post-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. What is the product of lipase hydrolysis?1 From your results, which pH is ideal for pancreatic lipase digestion?2 Which tube confirms that there is no lipase in bile salts or vegetable oil?3 Where in the body does pancreatic lipase hydrolyze triglycerides?4
Review Sheet Results Explain why you can't fully test the lipase activity in tube 5 (lipase, vegetable oil, bile salts, pH 2.0 buffer).You did not answer this question.1Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible reasons why it may or may not have matched.You did not answer this question.2Explain why pancreatic lipase would be active in both the mouth and the pancreas.You did not answer this question.3Describe the process of bile emulsification of lipids and how it improves lipase activity.You did not answer this question.4
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- Fall '19
- Nutrition, Lipid, session ID, session-2a304f16-ccb2