Procedure 1 each clan gets one 1 patient rectal swab

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Procedure 1. Each Clan gets one (1) “Patient” Rectal Swab Sample Plate. 2. Streak an EMB Plate from an isolated Colony on your Patient Plate. 3. Streak a Nutrient Agar Plate from an isolated Colony on your Patient Plate. 4. Incubate your Clan’s 8 Plates (4 EMB Plates + 4 Nutrient Agar Plates) at 37°C for 24 Hours. 5. Save your Patient Rectal Swab Plate at 04°C.
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Lab 1 Page 12 Rogues Gallery There are Thousands of different Bacteria -- would you have guessed that there are more than a Thousand different Bacteria just on your Skin -- but we ʼ ll be concentrating on the following eight (8) Bacteria in MIC 101. Bacteria found in Soil You ʼ ll be isolating these two Genera (Plural of “Genus”) from the Soil. They ʼ re very easy to isolate and purify, and they ʼ re comparatively harmless. Pseudomonas putida (Atlas Page 154) The Genus Pseudomonas contains Gram Negative, Obligate Aerobic Rods. They are also commonly found in Soil and most are Motile. Many Species of Pseudomonas can be coaxed into releasing fluorescent Siderophores (G= star/iron holders) to sponge-up Iron by growing them on Iron-Free Medium called F Agar (F= Fluorescence) . Lots of Bacteria produce Siderophores but only Pseudomonas produces Fluorescent Siderophores. And you can’t help noticing that our Pseudomonas putida is putting-on quite a Show. Pseudomonas putida is on F Agar. This is a Medium without any Iron. The Yellow-Green Color is due to Pyoverdin (G= green pus) a flourescent Siderophore characteristic of Pseudomonas . The Colonies are small and Clear-to-White. One of the most remarkable Physiological Properties of the Genus Pseudomonas is the very wide Range of different Organic Compounds that it can use as Sources of Carbon and Energy. Pseudomonas is one of the very few Bacteria that can utilize Benzoate as a Carbon Source. The Utilization of Benzoate requires Oxygen and Pseudomonas is Motile so it can migrate to the Surface of Non-Aerated Benzoate Media where the Oxygen Level is higher. These two Traits -- the Ability to utilize Benzoate and the Ability to migrate towards Regions of higher Oxygen Levels -- provide the Basis of our Pseudomonas Enrichment Strategy. A Soil Sample will be placed in a Test Tube of Medium in which Benzoate is the sole Carbon Source. This Test Tube will be left Stationary (Non-Aerated). This will soon result in an Anaerobic Environment at the Bottom of the Test Tube and an Aerobic Environment at the Air/Medium Interface at the Top of the Test Tube. Pseudomonas can migrate toward and remain in this Interface and utilize Benzoate in this Aerobic Environment. As a Result, Pseudomonas will frequently become the Dominant Bacterium in the Test Tube. _______________________ “Is there anything I can do?” Not unless you can alter time, speed up the harvest or teleport me off this rock.’ © 1977 Lucasfilm, Ltd.
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Lab 1 Page 13 Bacillus cereus (Atlas Pages 134 and 135) The Genus Bacillus contains Gram Positive, Facultative Aerobic, Spore-Forming Rods. They are commonly found in Soil. Many are Motile. Many Species of Bacillus
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