Coomassie brilliant blue agar CBB 001 wv coomassie brilHant blue dye CI 42655

Coomassie brilliant blue agar cbb 001 wv coomassie

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Coomassie brilliant blue agar (CBB) 0.01% (w/v) coomassie brilHant blue dye [CI. 42655] TSA Sterilise at 12rc/15min. Cysteine blood agar 0.3% (w/v) 0.1% (w/v) 20% (v/v) 0.5% (w/v) 0.05% (w/v) 1.5% (w/v) beef extract L-cysteine hydrochloride human blood sodium chloride yeast extract agar Cytophaga agar (Anacker and Ordal, 1959) 0.05% (w/v) 0.05% (w/v) 0.02% (w/v) 0.02% (w/v) 0.9% or 1.0% pH (w/v) tryptone yeast extract sodium acetate beef extract agar 7.2-7.4 SteriHse at 121°C for 15 min. Improved growth medium for Flu. psychvophilum (Daskalov et ciL, 1999) Cytophaga agar/broth supplemented with: 0.05% (w/v) D(+) galactose 0.05% (w/v) D(+) glucose 0.05% (w/v) skimmed milk powder The supplements were prepared as 10% (w/v) solutions, and filtered separately through 0.22 |im porosity filters, and added to molten, cooled medium.
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176 Bacterial Fish Pathogens Dorset egg medium 75% (v/v) fresh egg mixture (whites and yolks) 0.25% (w/v) Lab-lemco powder 0.25% (w/v) peptone 0.125% (w/v) sodium chloride Crystal violet may also be incorporated to suppress contaminants Inspissate (~80°C for 1 hour). Emerson agar 0.4% (w/v) beef extract 0.1% (w/v) yeast extract 0.4% (w/v) peptone 1% (w/v) dextrose 0.25% (w/v) sodium chloride 2% (w/v) agar pH 7.0 Sterilise at 12rc/15min. Flavobacterium columnare selective medium (Fijan, 1969) 0.02% (w/v) beef extract 0.05% (w/v) tryptone 0.05% (w/v) yeast extract 0.02% (w/v) sodium acetate 0.9% (w/v) agar pH 7.2-7.4 SteriHse at 12rC/15min, allow to cool, and add filter-steriHsed antibiotic solu- tions: 5 |ig/ml of neomycin sulphate and lOTU/ml of polymyxin B. FLP medium (for Flu. psychwphilum; Cepeda et al, 2004) 0.05% (w/v) glucose 0.02% (w/v) CaCl2.2H20 0.05% (w/v) MgS64.7H20 0.4% (w/v) tryptone 0.04% (w/v) yeast extract 1% (w/v) agar pH 7.2-7.4 Sterilise at 12rc/15min. Kidney disease medium 2 (KDM2) 1.0% (w/v) peptone 0.05% (w/v) yeast extract 0.1% (w/v) L-cysteine hydrochloride 1.5% (w/v) agar pH 6.5 SteriHse at 1 2 r c for 15min, cool to 45°C, and add 20% (v/v) sterile foetal calf serum.
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Isolation/Detection 177 L-F medium 1.0% (w/v) 10.0% (w/v) 0.5% (w/v) 1.0% (w/v) brain heart infusion sucrose yeast extract agar No. 3 Sterilise at 115°C/20min 10% (v/v) horse serum, inactivated by heating at 60°C/30min Cool the medium and warm the horse serum to ~50°C, mix and pour as plates. Loeffler (serum) medium 75% (v/v) horse serum 0.25% (w/v) Lab-lemco powder 0.25% (w/v) peptone 0.25% (w/v) dextrose 0.125% (w/v) sodium chloride distilled water to 11. Lowenstein-Jensen medium 0.15% (w/v) 0.15% (w/v) 0.037% (w/v) 0.22% (w/v) 0.73% (v/v) 1.83% (w/v) 60.97% (v/v) 0.024% (w/v) potassium hydrogen phosphate magnesium sulphate hydrated magnesium citrate asparagine glycerol potato starch fresh egg mixture (whites and yolks) malachite green Inspissate (~80°C for 1 hour) MacConkey agar 2% (w/v) 1% (w/v) 0.5% (w/v) 0.5% (w/v) 0.0075% (w/v) 1.3% (w/v) pH peptone lactose bile salts sodium chloride neutral red agar 7.4 Sterilise at 12rc/15min. Medium K (Mudarris and Austin, 1989) 0.5% (w/v) beef extract 0.6% (w/v) casein 0.2% (w/v) tryptone 0.1% (w/v) yeast extract 0.1% (w/v) calcium chloride 1.5% (w/v) agar pH 7.0 Sterilise at 12rC/15min.
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178 Bacterial Fish Pathogens Middlebrook 7H10 agar 0.05% (w/v) ammonium sulphate 0.15% (w/v) potassium phosphate 0.15% (w/v) sodium phosphate 0.04% (w/v) sodium citrate 0.0025% (w/v) magnesium sulphate 0.00005% (w/v) calcium chloride hydrated 0.0001 % (w/v) zinc sulphate 0.0001% (w/v) cupric sulphate 0.05% (w/v) L-glutamic acid 0.5% (v/v) glycerol 0.04% (w/v) ferric ammonium citrate 0.0001 % (w/v) pyridozine 0.00005% (w/v) biotin 0.000025% (w/v)
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