Rnas range from about 240 to 600 nucleotides 10 size

Info icon This preview shows pages 7–8. Sign up to view the full content.

View Full Document Right Arrow Icon
-RNAs range from about 240 to 600 nucleotides (10% size of smaller viruses) - No evidence that RNA codes for proteins; viroids use host enzymes & proteins completely - Example: duplication of viroid RNA in infected cell uses host RNA polymerase II q May cause disease by interfering with cell's normal path of gene expression (e. g. monopolize RNA polymerase II to duplicate viroid RNA) q Viroid diseases can have serious effects on crops - Cadang-cadang viroids of coconut palms (Philippines) - chrysanthemum viroid (U. S.) q Recombinant DNA Technology q Recombinant protein Expression q Microarray q Agarose Gel Electrophoresis q SDS-PAGE q Polymerase Chain Reaction q Immunoassays (Western Blot, ELISA etc.) q Site Directed Mutagenesis q Cell / Tissue Culture q Chromatography q Microscopy q Ultracentrifugation CD34+ cells Ability to differentiate into any type of cells First found in mammalian embryo in liver then spleen Less than 0.1% of all cells in the adult bone marrow Limited proliferative capacity (to maintain the population) Promising therapeutic potential Totipotent Stem Cells Can differentiate into any cell type in the body plus the placenta Zygote and its first few daughter cells (32 cell stage) Pleuripotent Stem Cells Descendants of totipotent stem cells Develop up to four days after fertilization (human embryo) Can differentiate into any cell except totipotent cells Multipotent Stem Cells Descendants of the pleuripotent stem cells and antecedents of specialized progenator cells Give rise to a family of related cells Ex: Hematopoietic stem cells and neural stem cells Unipotent Stem Cells (Progenator cells) Can differentiate only into one type of cell Ex. Erythroid progenator cells Human embryonic stem cells grown for 10 months in the absence of mouse feeder cells
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
8 q Give one potential application of Stem Cells in the therapy of human diseases. q Answer must be based on a journal article. q Summarize your answer in NOT more than 500-word essay. q Include the complete citation reference material used (not included in word count) q Submit printed copy of answer next meeting.
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern