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13) What is the purpose of the TBE buffer in this experiment?14) What is the purpose of the Ethidium Bromide in this experiment? What does it mean to call EtBr an intercalating agent?15) If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? a)The cell would be unable to replicate its DNA.b)The cell would create incomplete plasmids. c)The cell would be easily infected and lysed by bacteriophages. d)The cell would become an obligate parasite. 16) What is the enzymatic function of restriction enzymes ? 17) How does a bacterial cell protect its own DNA from restriction enzymes?18) During electrophoresis through an agarose gel, DNA migrates towards the This handout was modified from the Biotechnology Explorer (BioRad Labs) handout: “ Restriction Digestion and Analysis of Lambda DNA”, and a similar lab handout written by Dr. Jackie Reynolds at Richland College, Dallas TX.