From these results it was considered that the cultures resembled Ser plymuthica

From these results it was considered that the

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From these results, it was considered that the cultures resembled Ser. plymuthica and Ser. rubidaea (Grimont and Grimont, 2005a). Yet, as a result of acid production from inositol, but not adonitol, it was considered that the pathogen should be assigned to Ser. plymuthica. Similar traits were reported by Austin and Stobie (1992b). Yersinia intermedia Characteristics matched the species description (Farmer and Kelly, 1991) at 36°C, except that the fish isolate utilised sodium citrate and was weakly positive in the Voges Proskauer reaction (Carson and Schmidtke, 1993). Yersin ia in termedia The culture comprises fermentative, motile (at 25°C but not 36°C) cells that produced P-galactosidase and indole, but not arginine dihydrolase, H2S, lysine or ornithine decarboxylase or oxidase. Aesculin and urea are degraded. The methyl red test and Voges Proskauer reaction (at 36°C but not 25°C) are positive. Nitrates are reduced. Acid was produced from glycerol, inositol, man- nitol, melibiose (at 36°C but not 25°C), rhamnose, sorbitol, sucrose, trehalose and
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Characteristics of the pathogens: Gram-negative bacteria 111 xylose, but not adonitol or lactose. Sodium citrate is utilised at 36°C, but not 25°C (Carson and Schmidtke, 1993). Yersinia vuckevi Enteric redmouth (ERM, Hagerman redmouth disease, redmouth, salmonid blood spot) was initially diagnosed as a systemic infection among farmed rainbow trout in the Hagerman Valley of Idaho during the early 1950s, and subsequently described in detail by Ross et al (1966). Yersinia nickeri The organisms comprise a homogeneous group of fermentative. Gram-negative, slightly curved rods of 1.0 x 2.0-3.0 |im in size, which are motile usually by means of 7 or 8 peritrichously arranged flagella. Catalase, P-galactosidase, and lysine and ornithine decarboxylase are produced, but not H2S, indole, oxidase, phenylalanine deaminase or phosphatase. The methyl red test is positive, but not the Voges Proskauer reaction. Nitrates are reduced. Gelatin and Tween 20, 40 and 60 are degraded, but not aesculin, chitin, DNA, elastin, pectin, tributyrin, Tween 80 or urea. Growth occurs in 0-3% (w/v) sodium chloride. Sodium citrate is utilised. Acid is produced from fructose, glucose, maltose, mannitol and trehalose, but not from inositol, lactose, raffinose, salicin, sorbitol or sucrose. The G + C content of the DNA is 47.5-48.5 mol % (Ewing et ciL, 1978). The precise taxonomic position of the causal agent of ERM has intrigued bacteriologists since the initial isolation of the organism. Ross et al. (1966) reahsed that heated O-antigens prepared from 14 cultures of the ERM organism agglutinated strongly (titre = 1:320 or 1:640) with the corresponding antigens of Sal. enterica subsp. arizonae O group 26, and weakly (titre = 1:20) with O group 29. Conversely, there was no reaction with O-antigens prepared from Salmonella, Ent. liquefaciens, Citrobacter or Serratia. In addition, this team pointed to the biochemical similarities with Ent. liquefaciens, Ser. marcescens subsp. kiliensis and Sal. enterica subsp. ari- zonae. Furthermore, Stevenson and Daly (1982) indicated serological cross-reactions with Haf. alvei.
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