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1 the mechanism of action of restriction enzymes 2

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1. The mechanism of action of restriction enzymes. 2. The different results you would expect if a mutation occurred at the recognition site for enzyme Y. 2002: Information transfer is fundamental to all living organisms. For two of the following examples, explain in detail how the transfer of information is accomplished. a) The genetic material in one eukaryotic cell is copied and distributed to two identical daughter cells. b) A gene in a eukaryotic cell is transcribed and translated to produce a protein. c) The genetic material from one bacterial cell enters another via transformation, transduction, or conjugation. 2002: The human genome illustrates both continuity and change. (a) Describe the essential features of two of the procedures/techniques below. For each of the procedures/techniques you describe, explain how its application contributes to understanding genetics. The use of a bacterial plasmid to clone and sequence a human gene. Polymerase chain reaction (PCR) Restriction fragment length polymorphism (RFLP) analysis (b) All humans are nearly identical genetically in coding sequences and have many proteins that are identical in structure and function. Nevertheless, each human has a unique DNA fingerprint. Explain this apparent contradiction.
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